What is PCR amplification protocol for iQ-Check Quanti L. pneumophila Kit?

Purchased kit to run on old iCycler but directions did not have a method. Only said to use CFX Manager Software and select the appropriate thermo-protocol for the detection or quantification of...

13 November 2020 8,937 1 View

Does anyone have experience giving a CSF-1 R inhibitor via IP injection to deplete microglia?

Dear colleagues, I am studying microglia in early development. I would like to design a study where I deplete microglia in neonatal pups using a CSF-1R inhibitor. Several studies that employ the...

06 November 2020 793 2 View

Best fluorescent antibodies for whole-mount staining of mouse tissues?

Hello, I'm a first-year graduate student, and my lab is looking to get into whole-mount immunostaining of cleared mouse tissues (specifically, at this point, lung and skin). I'm very new to this,...

22 September 2020 8,582 3 View

Can anyone suggest some links where i can get free access to Synthetic Aperture Radar Images for research purposes?

Can anyone suggest some links where i can get free access to Synthetic Aperture Radar Images for research purposes?

20 December 2019 6,961 4 View

Can someone suggest or refer an effective procedure for the removal of a sample from Wax, so that it can be embedded in EPON for SEM?

We require a method that will keep the sample in tact while removing it from Wax embedding, so that it can then be embedded in EPON in order to avoid damage to the instrument during scanning...

19 November 2019 9,851 2 View

How do you best store isolated exosomes?

I am isolating exosomes from the SBI Exoquick TC Ultra kit. I'm going to run some right away on western blots, but was hoping to store some for a while to add onto gelatin matrices for imaging....

03 October 2019 6,254 3 View

What is the procedure required to get access to the publicly available MSTAR dataset?

What is the procedure required to get access to the publicly available MSTAR dataset?

31 March 2019 726 1 View

Before anything else..Thank you! I actually want to do a research on positive psychology.. Any interesting topic as your suggestion?

I want to do a research on positive or even in existential psychology that may add to my learning the field of psychology.. I just want to source out topics from experts like you. Thank you.

19 January 2019 9,698 6 View

What is as effective method to teach quick and accurate ELISA plate loading?

My new tech is so slow at adding saliva samples to competitive ELISA plates. I thought after a week of loading two full 96-well plates a day he's be more comfortable but he's using two hands to...

16 June 2018 2,404 3 View

Any recommend journals for social media influence voting behavior and perception?

Hi there, I'm currently working on my thesis on the topic of "How social media influence voting behavior and perception of youth". Hope to get some recommendation of journals to work out on this...

16 December 2017 4,030 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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22 February 2021 9,932 11 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View