Hi folks! I'm trying to stain for Tfh and B cells on PP for confocal microscopy but I don't have a good protocol. Also, is it better to use OCT or paraformaldehyde/sucrose? A working protocol would be appreciated! Thanks a lot!
I would like to perform functionalization using silanes on an already synthesized hydrogel crosslinked with citric acid. Does anyone know a methodology using solvents?
28 April 2024 6,747 4 View
Hola. Tengo una duda. Un artículo mío donde soy primer autor sale en la citación con el nombre de otro de los autores como primero, sin embargo en la revista no sale así. Al buscar en google...
20 September 2023 5,438 0 View
Hello, we are trying to standardize comet assay at our lab, however, on the visualization step, we don't observe any comets (or stained DNA nucleoids in the negative control slides). I attach to...
19 March 2023 5,499 0 View
I am looking for knowing the levels of body fat (BF%) estimated by 4 skinfolds (biceps+tricipital+subscapular+ suprailiac) in children aged 6-12y . Thanks!
15 January 2020 8,555 7 View
I want to carried out a study of karyology in cuban cactus and I would like to know advice from experienced people in this field
31 October 2019 6,196 3 View
I'm performing hydrothermal synthesis at 180C/2 hours and safety is questioning my procedure since the stainless steel top loosens some after cooling down. I've followed procedures I've seen...
06 August 2019 7,574 2 View
The ITD´s are the Decentralized Technological Institutes
22 September 2018 4,875 0 View
Someone could recommend any industrial antibodies against these glutamate receptors based on self experience? I am just looking for using in Immunoblot or Western.
22 March 2018 2,852 1 View
Using independent-culture approach, I have detected abundant 16S sequences belonging to Caulobacter sp. from agricultural soil samples, but I couldn't be able for isolating it using media like TSA...
07 August 2017 3,664 4 View
02 August 2017 6,171 3 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 640 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,708 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,892 1 View