Which are the normal levels of body fat in children estimated by anthropometry?

I am looking for knowing the levels of body fat (BF%) estimated by 4 skinfolds (biceps+tricipital+subscapular+ suprailiac) in children aged 6-12y . Thanks!

15 January 2020 8,376 7 View

Efficient antibodies against Gria1 (GluR1) and/or Grin2a (GluN2a)?

Someone could recommend any industrial antibodies against these glutamate receptors based on self experience? I am just looking for using in Immunoblot or Western.

22 March 2018 2,761 1 View

Isolation of Caulobacter from environmental samples. How?

Using independent-culture approach, I have detected abundant 16S sequences belonging to Caulobacter sp. from agricultural soil samples, but I couldn't be able for isolating it using media like TSA...

07 August 2017 3,572 4 View

Isolation of Caulobacter from environmental samples. How?

Using independent-culture approach, I have detected abundant 16S sequences belonging to Caulobacter sp. from agricultural soil samples, but I couldn't be able for isolating it using media like TSA...

02 August 2017 6,077 3 View

Will bioactive pure protein/peptide isolated from marine tissue show two consecutive peaks in HPLC?

The pure bioactive protein/peptide isolated from marine tissue in ion exchange column is showing two peaks in HPLC but a single band in SDS PAGE. How i can take these results? Whether the isolated...

16 February 2017 8,697 3 View

How can you succesfully integrate UDG enzyme digestion in to a LAMP protocol to eliminate carry-over contamination?

I have been trying to use the UDG enzyme in my LAMP protocol but I can't seem to find a way to successfully eliminate the false positive results.

23 January 2017 5,443 1 View

Are there any service or web site where other scientists can review research projects before applying?

04 May 2015 5,973 0 View

How do I get a results table in ANSYS using design assessment combinations?

I am trying to get the table of results of a combination of static and response spectrum, I would like to analyse the seismic effects and make some combinations based on design codes. Do you have...

01 February 2015 4,057 3 View

Should the response spectrum results (ansys) be higher than static structural analysis (i.e. stress)?

I've been working on a filled tank in Ansys structural, and the response spectrum analyses show me results smaller than static previously loaded. I checked the specrtum and it has no errors, but...

20 January 2015 2,198 1 View

Which way is the best to judge mesh quality in Ansys Mechanical?

12 January 2015 3,745 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View