I'm looking for one Ab targeting CD44-HA interaction and one that doesn't. The aim is to use the Abs to disrupt CD44 adhesion/signaling in an in vitro tumor cell culture, eventually leading to further in vivo studies adminiestering Abs to mice. Alternatively, how feasible would be the use of hyaluronidases or HA oligosaccharides to inhibit CD44-HA interaction? In the case of HA oligosaccharides, which one(s) would be best as inhibitors?
Rat mAbs IM7 and Hermes-1 are supposed to neutralize CD44. We list a couple of sources for both clones:
http://www.linscottsdirectory.com/search/antibodies/searchStr:CD44
Just select "Neutralization" from the Applications filter. Follow More Info to suppliers' pages.
Hope this helps.
CD44 standard isoform withour variable exons (predicted M.W. is about 75kDa) is known to highly ind to hyaluronic acids as compared with CD44 variant. As mentioned above, clone IM7 is best recognized to block the interaction of CD44 and ECM.
http://journal.frontiersin.org/article/10.3389/fimmu.2015.00201/full
Thanks very much for the answers. Based on the fact that my cells mainly express the CD44s form and on some literature search on the phenotype I'm looking for, finally I've decided to go for the Hermes-1 clone. Let's see if it works well.
Jordi Senserrich Does Hermes-1 clone inhibit the interaction between human CD44 and HA??
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