Which are the areas that have an edge for future funding within Cancer, Immunology and Epigenetics?

More Vimal Keerthi's questions See All

What is meant by Hits (DB) in High-Resolution Liquid Chromatography-Mass spectrometry (HR-LCMS) analysis compound table report?

In the qualitative compound report obtained from HR-LCMS analysis of crude plant extracts, what is meant by Hits (DB)? Should we consider all the predicted compounds from the list for further studies?

30 June 2024 2,290 4 View

Can 0.1% ammonium hydroxide damage HILIC column?

In my LC-MS experiment, I am utilizing an Agilent Polaris NH2 column to separate a mixture of glucose, fructose, and sucrose. I am using water and acetonitrile as A and B mobile phases...

26 March 2024 8,895 4 View

Can ProSA web server be used to analyze the protein structures downloaded from Protein Data Bank?

Or is it used only to analyze the modelled protein structures.

02 March 2024 6,662 2 View

How to visualize interactions of protein-protein docked model obtained from ClusPro web server?

Not able to view the downloaded model from ClusPro using PDBSum. Since both the docked proteins contain single A chain, PDBSum shows it as single image. Please give suggestions. Also tried to save...

18 January 2024 5,286 4 View

Why am I getting Parsing error when I upload an article?

Why am I getting a Parsing error when I upload an article? How do I fix it?

27 December 2023 3,159 6 View

Searching for a suitable Host institution to collaborate for research IN Dry eye Disease?

I am searching for a suitable workplace to collaborate for further research for the following schemes: 1. Institutions that can host our research for a short period of 6 months. Under the SERB...

14 October 2023 4,086 0 View

Why polymer like peaks with C30 column always?

I am using a C30 column for carotenoid analysis. I bought the column recently and using it for the first time. When I use the column in LC-MS scan mode, I clearly see the UV-Vis peaks for my...

08 June 2023 2,357 2 View

What is the possible peak at m/z = 535.8 for lycopene?

I am analyzing Lycopene using LC-MS. I use a C18 column and my mobile phase is Methanol: MTBE = 95:5. I use an Agilent Ultivo QQQ mass spectrometer with APCI negative ion mode. I see the precursor...

07 May 2023 525 0 View

How to get y+ value in Ansys CFX ?

I am working on simulation of savonius hydrokinetic turbine in cfx. I have set inflation layer on turbine as shown in photos. It is observed in the previous investigation that, the non-dimensional...

23 April 2023 2,983 3 View

What does "defined levels are outside of dataset range" mean in 2D NMR?

I am running an HMBC experiment on a Bruker 300 mHz instrument. I set up the experiment following the steps given in TopSpin guide book. Everything happens fine but when I try to open the spectrum...

09 March 2023 5,289 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Samrat Das

Dear Vimal,

Your question is very broad. The answer will also partially depend on your interest and your future research goals. Do you want to say that in " Cancer, Immunology and Epigenetics" which is the good choice ?.

Vimal Keerthi

Samrat Das Thank you for our response Mr. Das. More specifically I wanted to know about Caner and Immunology, and which areas of therapeutics would be more beneficial for the future.