How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View

How to measure the hardness for PLA & PHAs polymers?

Hello, I am a bit confused on whether to follow ASTM D785 (Standard Test Method for Rockwell Hardness of Plastics and Electrical Insulating Materials) or ASTM D2240 (Standard Test Method for...

21 January 2021 7,487 4 View

How to create suspended particles flow and simulate distribution in a chamber using CAD software?

Allow me first to apologize in advance for the long post. I have tried to keep it right-to-the-point as possible!. Objective To simulate suspension of solid particles using CAD software/create...

12 January 2021 6,389 2 View

How to properly purge an injection molding machine?

Hello, We're trying to purge a mini injection molding machine after not using it for a while now. The resin inside is PHA and can be some PLA left over as well. We've tried using a purging...

08 October 2020 4,659 5 View

How we could modeling orthotropic membrane elemnt in SAP2000?

I am trying to model steel plate shear walls in SAP 2000. First I should define an orthotropic steel material and define nonlinear parameter of it ( Stress-Strain curvature) in somehow to neglect...

22 May 2020 4,250 0 View

Hi,What is the names of all organic laser dyes?

Hi,What is the names of all organic laser dyes?

16 April 2020 3,543 11 View

Test aluminium foil If it has traces of rolling oil that could affect the coating adhesion?

I have to work with Aluminium foil (8079alloy- 38 micrometer thickness) , in the last months we encountered an adhesion problem between the aluminium and the coating materials (primer), so I think...

10 March 2020 2,739 10 View

How to calculate the surface area of the nanoparticles mathematically?

Please answer with equations or research that prove this

06 March 2020 7,428 3 View

Diabetes Locus of Control Scale?

Hi everyone. I am conducting a research study to explore the relationship between emotion-related variables such as locus of control and diabetes-related outcomes. However, I am not able to find...

29 February 2020 7,814 3 View

How to determine the initial phase angle in the SRF-PLL?

Dear all, My question is regarding the synchronous reference frame phase-locked loop (SRF-PLL), as discussed in the book "Doubly fed induction machines:Modelling and control for wind energy...

26 November 2019 1,829 5 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

What are the naitve functions of bacterial NRP?

The bacterial NRPs (nonribosomal polypeptides) are famous as clinical drugs such as antibiotics, but is there other native function of these NRPs of bacteria except antimicrobial, because their...

01 February 2021 4,280 1 View

How to calculate the CFU/ml for Mycobacterium tuberculosis growing in 7H9 broth?

I am running an experiment in which I need to calculate the CFU/ml of a Mycobacterium tuberculosis growing in 7H9 broth. Is there a reliable method to calculate cfu/ml for MTB since a culture of...

22 January 2021 10,027 2 View

How to revive bacteria from glycerol stock?

19 January 2021 7,025 4 View

How to extract and purify disaccharide peptides and lactoyl peptides from Mycobacteria cell wall?

Recently, I try to obtain disaccharide peptides and lactoyl peptides from Mycobacterium Smegmatis, but I cannot succeed. My method is: Disaccharide peptides: The mycobacterial strains were...

12 January 2021 8,352 3 View

Does anyone grow Mycobacteria in TECAN Spark machine to get growth curves? What setting is necessary? Any tips (use Tween20 or tyloxapol?)?

I would like to grow Mycobact. Smegmatis in 24well plate in TECAN Spark machine. So far, I was using 100ml erlenmeyer flasks with 20 ml bacterial culture w/ shaking 180 rpm in 37C . Tecan would...

10 January 2021 6,624 2 View

Why I am not getting cultures of DH5 alpha and BL21 cells after transformation?

Even if I get them cultured colonies are very less and almost not transferable and grows very slowly in the media. I checked cells by transforming with control vector. I changed antibiotic also...

06 January 2021 1,013 3 View

Does anyone know one antibody that works against Mycobacterium spp?

Anti-Mycobacterium antibody: does anyone know one antibody that works against Mycobacterium spp. (mainly for immunofluorescence)? Many thanks in advance! Cristiane

04 January 2021 9,724 3 View