Which amino acid sequence methods are available while no MS is present?

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Adam B Shapiro

Sequencing from the C-terminus by carboxypeptidase digestion is described here:

http://www.ncbi.nlm.nih.gov/pubmed/18429238

Udaya Kiran Marelli

Hi Jordy Kocken,

If it is just a peptide of 3 amino acids, you can get the structure by NMR.

Steven Verhelst

Depending on the amount you have (anything from 1 mg up should be OK) and especially the purity, you should go for 1H NMR and 1H COSY if desired.

Jordy Kocken

Thank you for all the answers! We now have a working setup and are going to get some control samples running.