We are going to overexpress neurotrophic factors in the striatum and substantia nigra region of mice. We are interested to have overexpression in both neurons and glia. The viruses are intended to be administered sterotactically.
I'm not sure about glial cells, but according to my knowledge, AAV2, AAV5, and AAV-DJ seem to be the most widely used for neurons.
Thank you for your answer. What do you think about AAV1, AAV9 and PHP.eB?
Dmitriy Komkov I am not aware of PHP.eB, and I'm also not sure about serotypes 1 and 9. Still, according to previous literature, AAV9 has been widely used for cardiac cells and probably for neurons as well, but you would have to check other references. Another best option is to directly contact companies that offer virus packaging services and inquire about the transduction efficiency of each serotype.
Several AAVs are known to affect presynaptic release regardless of what their cargo is (namely AAV1, 5, 8 see Jackman et al., doi: 10.1523/JNEUROSCI.4694-13.2014). AAV9 was the exception and transduces neurons well so after that publication many completely stopped using 1, 5 and 8. PhP.eB and PhP.AX are newer variants that cross the blood brain barrier to transduce most of the rodent brain after i.v. administration. So if you want this route as an option choose one of them - just they don't work in every mouse strain! It is always a caveat that there could be 'side effects' of transduction. For PhP.eB and PhP.AX we haven't seen any but that might be due to a failure to look in the right place! We haven't done a Jackman-type study of release for instance. Also quality of AAV preps can vary - if you see a lot of microglial activation it might be the quality and not due to the AAV per se...
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