Christine E Gee

4 Questions 14 Answers 0 Followers

Questions related from Christine E Gee

Can someone recommend a far-red NIR calcium indicator (dye or GECI) that works well?

Have always used Green or red but now need something a bit further red.

10 October 2023 2,054 0 View

Can anyone point me to a 3D EM reconstruction of olfactory bulb external plexiform layer neuropil?

What I hope to see is the arrangement of astrocytic processes around the reciprocal synapses between mitral and granule cells. Similar to what has been done in cortex/hippocampus by various groups.

28 April 2023 1,026 0 View

Does anyone have a recipe for a photostable medium (e.g. Bright Cell Neumo/Memo, Fluorobrite) for brain slice cultures? (not dissociated cells)?

We want to perform long-term fluorescent imaging (12-24 hours) and reduce background fluorescence from Phenol red and serum components. We find recipes for dissociated neurons but so far none for...

02 August 2022 2,460 0 View

Can the sucess of recombination with FLEX/DIO constructs easily be determined?

We have a strange result: We transduce neurons with an AAV vector with synapsin promoter then DIO-ChrimsonR-mRuby - meaning there are double loxP-lox2722 sites flanking the inverted...

24 March 2022 1,362 2 View