How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View

How can I control Asphodelous tenuipholius in chickpea and mustard crop. Any effective post emergence herbicide?

In Bundelkhand region of Central India, Asphodelous tenuipholius is major problamatic weeds on farmers field. Any suggestions from weed scientists

29 November 2020 1,207 4 View

Effect of excess cyclodextrin on drug after cyclodextrin reaches maximum solubility in phase solubility study?

I am working on phase solubility studies between beta-cyclodextrin and drug? I used more concentration of beta-cyclodextrin till 30mM despite its maximum solubility of 16mM. Usually, above maximum...

30 October 2020 3,921 9 View

How to overcome the convergence problem in comsol nonlinear static analysis?

03 October 2020 3,834 3 View

Any tdTom antibody that can detect tdTom protein and also withstand citrate buffer antigen retrieval?

Hi, Our lab started using tdTom mice for lineage tracing. Currently we are using 2 antibodies to detect tdTom one from Clontech and other from rockland. When we use tdTom antibody for...

29 August 2020 4,105 2 View

Is the antenna designed for Ku band operating at 14.25GHz suitable for terrestrial communication? or suitable for RADAR communication?

I have designed an antenna to operate at 14.25 GHz( 14 to 14.5 GHz)uplink frequency of Ku band with linear polarization. what kind of application is suited for my designed antenna? and reference...

11 July 2020 699 2 View

Can any one help me with the MATLAB code for optimizing SHE PWM and NLC PWM with GA tool ?

Sir Iam doing MATLAB SIMULINK implementation of Switched capacitor multilevel inverter with high and low switching frequency technique.so i need help in doing matlab coding for optimizing SHE and...

23 June 2020 7,407 2 View

We are facing rotten problem in kesar mango near mouth of it. What may be reason for that?

We are doing natural farming of kesar mango. This year we are facing rotten problem in mango near mouth of it. Last year it seems good. For this reason we are facing problem in selling if it....

02 June 2020 5,277 7 View

Dear Research...how to resolve this error in mat lab(2018a)...and simulation symbol is not availble in simulink?

Dear Research...how to resolve this error in Matlab(2018a)...and simulation symbol is not available for simulating ...pls say how to solve this problem in Matlab. Thanks you in...

19 April 2020 5,494 2 View

Any suggestions on the most useful publications/resources to teach undergraduates on "Ethics in Environmental Research"?

Will appreciate feedback from academics teaching this to students. Thanks.

05 April 2020 2,687 0 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View