Chet Ram

6 Questions 32 Answers 0 Followers

Questions related from Chet Ram

Why SSR amplification is giving Smeared, diffused and fuzzy bands?

Dear All I am trying to amplify the SSR markers on genomic DNA of date palm but it always gives smeared, diffused and fuzzy bands in gel electrophoresis. I have standerdized them as much as the...

02 February 2024 6,576 3 View

What is the frequency or rate of somaclonal variation in terms of percentage or per 1000 plants in tissue cultured plants?

17 March 2023 2,431 4 View

Whether perfect SSR markers are good or compound (imperfect) type?

I am trying to select microsatellite markers from the database for assessing genetic diversity and DNA fingerprinting for germplasm. The database has consisted of Di-, Tri-, Tetra-, Penta- and...

04 September 2021 1,355 4 View

Whether PCR product generated by degenerate primers can be sequenced directly?

I have designed degenerate primer pairs from heterologous systems and amplified in genomically less-explored plant species. Since degenerate primers amplify all possible related sequences [gene...

13 October 2018 4,309 2 View

Position of alternative spliced variants on the chrmosomes?

I have mined some gene families from a genome. I am getting some genes with high sequence similarities however they are residing at the different position on the chromosome. So my query is that...

09 March 2017 1,912 4 View

How can we detect transgene copy number by using only and only a tissue culture technique?

Suppose we dont have any other means of copy no. detection like blotting etc.

15 March 2014 1,113 2 View