Hi, I like to study high glucose affect in retinal endothelial cells, whether it is necessary to set experiment also with L-Glucose or Mannitol along with normal Glucose as Control.

1 2 3 4

Normal Glucose L-Glucose Mannitol High Glucose

Whether 1,2, and 3 will be my Control against High Glucose ?

Or just Normal glucose act as Control?

Like to have your valuable suggestion.