Koushik Mondal

14 Questions 12 Answers 0 Followers

Questions related from Koushik Mondal

Could anyone please share the protocol to differentiate Th1, Th2, and Th17 T cells from the PBMC?

I need the protocol for differentiating the Th1 Th2, and Th17 and Treg cell from the isolated PBMC from human blood. Please help me with the protocol. Thanks, and regards.

19 February 2025 4,671 0 View

What is digital pathology slide scanner?

Could anyone please provide me with the information on digital pathology slide scanner? Having computer programming software.

24 April 2023 8,990 1 View

Are there any mouse cell line of Gastric adenocarcinoma?

Please suggest me with mouse gastric adenocarcinoma cell line to use it in B6 mice to develop cancer.

14 February 2023 5,521 0 View

Are there any specific mouse model for study of Rectal carcinoma?

I will be happy to know which mouse model is best for the study of rectal carcinoma. There are different mouse model to study colorectal carcinoma, but I want specifically the rectal...

18 January 2023 8,330 2 View

Can CCR4 and CCR8 be generalized with its association with hot tumor and cold tumor, respectively?

I like to know whether, we can generalize the association of CCR4 with immunologically active hot tumor. Alternatively, CCR8 with cold tumor.

14 October 2022 431 0 View

How can I insert and treeat cells with Ceramide and Ceramide1-phosphate?

It will be great if someone helps me with the protocol to treat cell lines with Ceramide and Ceramide-1-phosphate. I heard BSA and liposome-mediated delivery, looking for the protocol following...

09 June 2021 406 3 View

Whether I need to add L-Glucose or Mannitol in my cell cuture experiment to understand High glucose effect in endothelial cells?

Hi, I like to study high glucose affect in retinal endothelial cells, whether it is necessary to set experiment also with L-Glucose or Mannitol along with normal Glucose as Control. 1...

02 July 2020 1,056 2 View

How can I identify some specific subset in murine splenic B cell population behaving as regulatory B cell?

I want to know about the specific surface marker protein to identify an effector cell behaving as regulatory B cell that secrets cytokines in mouse. whether it is CD5, TIM1 or CD1d or any other...

02 July 2016 7,167 1 View

Could anyone provide me Glucocorticoid resistant Acute lymphocytic leukemia cell line CEM-C1-15 and sensitive cell line CEM-C7-14 ?

I like to work on Glucocorticoid response on Acute lymphocytic leukemia, could anyone provide me GC resistant cell line CEM-C1-15 and sensitive cell line CEM-C7-14

06 January 2016 5,396 2 View

Can anyone recommend me the catalog number of CpG-ODN to stimulate mouse spleen purified B cell to identify IL6 secretion?

I want to know about the company and the catalog number of mouse CpG-ODN to stimulate mouse purified B cell for IL6 secretion

17 November 2015 5,771 5 View

What is the infection protocol by leishmania donovani promastigote in MACS purified splenetic B cell?

Could anyone suggest how I can infect MACS purified B lymphocyte by promastigote of leishmania donovani? I am trying it with RPMI+10% FBS, keeping it for 24hrs incubation. Like to have your...

10 June 2015 7,410 9 View

How much splenocyte/lymphocytic cells do I need for 6 well/12 well /24 well for B cell mediated IL-10 secretion assay standardization?

I want to observe IL10 secretion by lymphocytic cells, could anyone of you guys suggest to me how much splenocytic/lymphocytic cell I need to plate for 6 well/12 well/24 well plates

17 March 2015 2,225 6 View

How I can measure membrane-bound IgM and IgG and secretory IgM and IgG?

I want to observe membrane-bound and secretory IgM and IgG. Could anyone provide me with the protocol?

16 October 2014 3,812 2 View

How can I measure the lambda kappa ratio during autoimmunity?

I want to identify the loss of receptor editing during a Leishmania infection, that's why I like to understand the lambda kappa ratio. Could anyone provide the protocol for this experiment, please?

14 October 2014 6,559 3 View