I have purified PEG modified albumin by ion exchange chromatography. Final product 96KD analyzed on analytical FPLC with >99% purity. 

My PEG reagent if 30KD and Albumin is 66 KD, after conjugation the total mass of PEGylated albumin is 96 KD. When I analyzed by MALDI TOF, I observed two peaks, one is 96 KD and the other is 66 KD. 

Why the 99 % pure modified protein product shows two peaks ?

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