I have to go through in-vitro Cell uptake study for which I need to load the nanoparticles with 6- Coumarin. Therefore, I want to know which 6-Coumarin can be used.
Probably so. But the dye is pretty exotic, its excitation and emission profiles do not fall within standard microscopy filters. Why not use a cyanine?
If you prefer Coumarin 6 for some reason, HPLC grade is probably the best that you can find. BTW do you need to load it non-covalently? It does not have any reactive group to bind it chemically.
Yep, still fluoresces after 40 years. But be sure you don't quench the fluorescent signal with something like Methanol.
http://www.sigmaaldrich.com/catalog/product/aldrich/546283?lang=en®ion=US
Thanks a lot to Sir Ustinov and Sir Neagle.
Sir, i have to load 6-coumarin in drug containing plga nanoparticles formulated by emulsion solvent evaporation technique.
http://www.sigmaaldrich.com/catalog/product/sigma/c4261?lang=en®ion=IN
Whether the above mentioned 6-coumarin grade can be used.
Dipali,
You are using the wrong molecule. You're not citing Coumarin-6. Look at the structure!
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