How can I use Permanova to analyze individual variation with RNA seq transcripts and multiway Anova design?

I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...

13 February 2021 7,217 4 View

Does anyone know of an anti-rat CLEC-2 antibody for IHC?

I am looking for an anti-rat CLEC-2 antibody for IHC in rat lungs. Has anyone come across one? There are a few mouse ones but the data sheets don't say they cross react with rat, and the ones that...

06 December 2020 3,511 0 View

Help finding right expression to fit data?

Hello, I have some data that looks like the line on the right, but I'm not sure what function to use. It seems like an exponential added to a line, or something of the like. I think it may be the...

18 November 2020 2,357 3 View

Single-turnover kinetics question?

It is my understanding that single-turnover reactions isolate the chemical step, as opposed to being comprised of the chemical step + product dissociation as in kcat. Here's my issue though....

13 November 2020 2,494 7 View

Which procedure would be the most successful to determine glomalin in soil?

We have to determine easily-extractable glomalin (EEG) and total glomalin (TG) from soil samples, but we have found various contradicting methods and calculations. Is there a trusted method that...

20 October 2020 3,051 3 View

Contrasting singular means?

Does anyone know of a method or approach in SPSS to contrast singular mean scores of overall samples, based only on the overall mean value, standard deviation figure and sample size? The data is...

29 September 2020 5,872 6 View

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09 July 2020 9,914 1 View

What kind of qualitative method to use?

Dear research colleagues, I am currently working on a qualitative research and would like to ask your opinion. I am not sure which qualitative method to use. We conduct semi-structured interviews...

14 April 2020 5,777 17 View

What are some good recommendations for free software that could be used to quantify video data on juvenile fish behavior and swimming activity?

Ideally I would like to use free software that can be used with a Macintosh. My objective is to quantify individual swimming patterns from 2-dimensional video data. I would like to collect...

16 March 2020 7,635 3 View

How can teacher education reflect the pedagogical ideal of democratic schooling based on dialogue?

04 March 2020 1,801 16 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View