Where to order PNA in the UK?

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Statistical test for weight data: 2 different groups overtime? 2-way repeated measure ANOVA?

Hello, I have weights over 5 weeks for 2 different groups (the animals in group A are different from animals in group B). I have thought to use a 2-way repeated measure ANOVA to see if there...

08 July 2024 1,163 3 View

CRAFT 21 Recogntion?

Is there a CRAFT 21 recognition trial?

14 March 2024 8,065 1 View

Potassium Permanganate titration- more hydrogen peroxide detected than what is actually present in solution?

I have been performing titrations of a known concentration of hydrogen peroxide using 0.01N potassium permanganate and consistently requiring more potassium permanganate than what is estimated to...

07 February 2024 446 3 View

Eye-tracking experiment using touch-screen/monitor with young children?

Has anyone used (or attempted to use) a touch-monitor or a touch-screen-laptop for the recording of behavioral responses in an eye-tracking experiment with young children? This would be ideal...

24 January 2024 6,584 4 View

Is Raman spectroscopy available for carbide/carbonitride detection in the ferrite steels?

My goal is to determine the volume fraction of M23C6 carbide and/or MX carbonitride in the ferritic/martensitic steels. Could I use Raman spectroscopy for this goal? If yes, are there the positive...

21 January 2024 9,585 2 View

How can I compare categorical datasets with many factors in R (e.g., polymer types at different locations)?

I want to know if the type of litter found between sites is significantly different. I have 7 stations with polymer IDs for litter from each station. Can I compare two categorical variables...

19 September 2023 6,208 3 View

What impact does the use of artificial intelligence have on the accuracy and speed of laboratory testing in clinical settings?

Medical laboratory technologists are likely to be faced with the challenges brought on by Artificial Intelligence (AI) as laboratory medicine continues to undergo digitization and automation. The...

02 September 2023 6,162 3 View

How many data extracts required for a qualitative dissertation?

I'm currently writing my qualitative doctoral dissertation and I'm not quite sure how many extracts do I need to analyse? Are 30-40 extracts enough for a dissertation?

04 July 2023 3,127 4 View

Tips to improve transfection efficiency?

Hello, recently I’ve transfected some HEK-293T cells with Thermofisher’s Lipofectamine 3000 reagent, but I’ve only gotten a transfection efficiency of about 20-30% using 7.5 uL of lipofectamine. I...

23 June 2023 4,965 1 View

Hello! Is there a chance glucosamine and galactosamine could be separated with C18 column via HPLC?

Hello! I‘m a student and the topic of my research is developing a method for quantitive analysis of glucosamine and chondroitin (in tablets after dissolution) via HPLC-DAD. We‘ve been using...

11 May 2023 2,354 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Identify the eight key processes of excellent supply chain management and discuss how each of these processes impacts the end customer?

Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26

04 August 2024 9,919 4 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Ayoob Obaid Alfalahi

Why you don't try to contact the supplier and they will guide you?

https://www.pnabio.com/products/PCR_blocker.htm

Best Luck.

Mushtak T. S. Al-Ouqaili

You will find all the information you need on the supplier page on the web!

Regards.

Hassan Nima

this link is useful

http://www.pna.co.uk/

regards