Does cleaning up cDNA after reverse transcription reduce late cycle background?

I've been wondering if removing the random primers from the reverse transcription reaction either with a column or precipitation before using it as a qPCR template reduces the background a bit in...

25 December 2020 6,258 4 View

Are you a virtual team member/leader who would like to participate in a Delphi study on International Online Collaboration Competencies (IOCC)?

You are invited to participate as expert panel member in our consensus study on International Online Collaboration Competencies (IOCC). The purpose of this study is to build consensus on the key...

11 November 2020 9,715 1 View

Bonferroni or Tukey: Which post-hoc test is adequate for my data?

Hello, I am running an ANOVA between 3 groups (Group 1 N=148, Group 2 N=147, Group 3- N=144; equal variances assumption met) . I am having difficulties in deciding upon the most adequate post-hoc...

21 October 2020 2,182 5 View

Does anyone know a good paper about deep watershed application for XCT images of rock?

I was able to find some applications of deep watershed for image instance segmentation, but nothing for rocks. Could you please recommend any study?

07 September 2020 5,731 8 View

What are reaction conditions for aromatic sulfonation of a dibenzyl ether derivative?

I am using chlorosulfonic acid in chloroform (inert, 2 hr, RT) to para-sulfonate benzyl groups on a dibenzyl ether. The work up is extraction with water. The reaction, however, does not seem to...

04 August 2020 4,446 7 View

Can you recommend any publications or authors on ethical and eco-friendly marketplaces?

Investigating the difference in business strategies between ethical marketplaces and traditional online marketplaces such as amazon

27 July 2020 7,302 3 View

What are your nanoindentation tips cleaning tricks?

Dear users of nanoindenters, I was wondering what procedures do you usually use to clean your diamond nanoindentation tips? I know that there is nothing specifically described but some internal...

08 July 2020 5,926 5 View

How good is the quality of spectra taken by portable NMR spectrometer?

At the moment our laboratory would like to buy portable NMR spectrometer and we would like to see some real not promo spectrograms. Could those who constantly work with such devices share spectra...

22 June 2020 8,144 6 View

What areas of the brain can be measured using EEG? What functions are appropriate and not appropriate to study with this method?

What areas of the brain can be measured using EEG? What functions are appropriate and not appropriate to study with this method? Can the EEG record accurately activity in deeper brain structures...

11 March 2020 1,087 6 View

Online testing solutions: any online tool permitting recording of participants' verbal responses?

Covid-19, apart from all health consequences, is also having an impact on research projects with human participants. Universities and schools are shutting down and, even when labs are still open,...

11 March 2020 2,711 16 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View