Mixed ANOVA - help?

I'm currently working on a piece which asks to conduct an ANOVA - looking at the effect of sporting group (2 levels), and exercise trials (two trials with six levels). I've computed this all...

27 February 2021 9,626 4 View

How to perform a mixed-effects Poisson regression with mutliple DVs?

I have a set of 6 related likert-type DVs, a within-subjects time variable (i.e. each DV was measured at 3 time points), and a categorical between-subjects IV (condition). Is there a way to...

16 December 2020 9,798 10 View

Repeated measures ANOVA with technical replicates?

I am doing an experiment where I measure the blood pressure of a treatment and control group over the course of several weeks. At each measurement session I collect multiple recordings. From what...

06 December 2020 531 7 View

Top-coded age and regression?

What is the best way to deal with top-coded age while doing regression analysis. If you are examining the presence or absence of a disease as your outcome, and a number of independent variables...

13 October 2020 907 5 View

Is there a guide that will help interpret Ponceau S staining for western blots?

Greetings, I'm having difficulty understanding my results from doing a ponceau S stain on a nitrocellulose membrane after western blot. I loaded 10ug in lanes 2,4, and 6. 15ug in lanes 3,5,and 7....

22 July 2020 4,616 1 View

Developing Calprotectin Assay for Bovines- extraction buffer selection and centrifuge settings?

Hello, I am trying to adapt the human Calprotectin assay to use in bovines. For background, Calpotectin is a protein released by neutrophils following gastrointestinal inflammation. A couple...

16 March 2020 1,261 3 View

Are there additional practice datasets with code for Multiple-QTL Mapping in R/QTL other than the tutorial on the website?

Multiple-QTL Mapping in R/QTL

20 February 2020 1,469 1 View

Thickest sections possible for slide-mounted IHC of FFPE mouse brain tissue?

I am currently trying to section FFPE mouse brains using a rotary microtome for fluorescence IHC. I'd prefer to collect the sections free floating in buffer, stain them free floating and then...

04 February 2020 6,858 1 View

Antibody recommendation for IHC anti-IL18 staining in mouse brain free floating slices?

Hello, Many papers i look at include the santa cruz M-19 antibody which is now discontinued. I did find a few papers listing the R&D antibody raised against rat (AF521) but I believe this...

02 December 2019 8,578 3 View

How do you analyze ELISA OD values to determine the best concetrations to work with?

I am developing and optimizing an ELISA and I was jsut wondering how you determine what the best concentrations of antibody and antigen are based off the OD values. I have performed checkerboard...

18 August 2019 7,599 3 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Why is there no protein expression but antibiotic resistance in transduced A549 cells with pLVX-TetOne-Puro lentivirus?

Hi, I have been trying to transduce A549 and Calu-3 cells using the Lenti-X Tet-One inducible expression system (TakaraBio). My gene of interest is inserted in a pLVX-TetOne-Puro plasmid and has...

15 February 2021 8,462 3 View

Is there a way in which microglia can be extracted from mice without killing them ?

I'm aiming to use an adoptive cell transfer approach, with microglia in mice with ALS. So far all the protocols I have seen, require sacrificing the mouse. I'd like to re-infuse the microglia back...

11 February 2021 9,509 1 View

Do you think NCC and 3 year service at army base must be compulsory to make the students loyal and discipline to the nation?

Decipline and loyalty for nation is the need of today.

09 February 2021 6,928 3 View

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

Professional editing and proofreading services?

08 February 2021 7,901 6 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

Would you like to participate in our study by completing the questionnaire?

04 February 2021 7,661 1 View

Choise of cell line for neurobiology?

I am working with myelin-related proteins and interested to do cell experiments with my target protein in cells. I have created lentiviruses for my proteins, and mutants and also CRISPR set-up for...

02 February 2021 8,083 1 View