Where to buy custom lentivirus?

More Lauren Herron's questions See All

How to culture and stimulating hamster splenocytes for T-cell analysis and ELISPOT?

Hi, Does anyone have experience and success of isolating, culturing and stimulating hamster splenocytes for T-cell analysis (FACS and ELISPOT)? I am currently using a protocol that we routinely...

09 July 2024 6,336 1 View

How to pellet adipocyte spheroids?

I am using the hanging drop method to develop individual differentiated adipocyte spheroids from the stromal vascular fraction of adipose tissue. With our current timeline they end up 100-300um....

27 May 2024 1,303 1 View

How can I increase transfection efficiency in my DC2.4 cell line?

I have been transfecting a dendritic cell line for about a year now with about a 15-30% transfection efficiency that I would like to increase to 40-50%. I have used Neon Transfection...

13 February 2024 8,526 4 View

Users or Past Users of Peyote and/or San Pedro?

I am a senior anthropology student at California State University Northridge (CSUN). I am looking for a few people who use/have used peyote/San Pedro for their health and/or religious/spiritual...

20 November 2023 6,616 0 View

How to prevent capillary clogging for zebrafish microinjections?

Hi all, I've been working on a project that involves microinjecting cells into zebrafish. A big issue has been needle clogging. Once capillaries are loaded, cells clog the tip of the needle and...

17 November 2023 8,670 4 View

How to reduce zebrafish mortality post injection?

I am doing an experiment involving microinjections with zebrafish and have been experiencing high amounts of mortality. The embryos look healthy upon injection, but many of them are dying between...

17 November 2023 5,510 2 View

Is transformation of agrobacteria with freeze-thaw method possible with large plasmid?

I have a large plasmid (20kb) which I am trying to transform into chemically competent commercial EHA101 cells. I had success with transforming a 16kb plasmid into the same strain, but have been...

07 August 2023 8,527 6 View

Can anyone recommend the best way to culture the Salmon Head Kidney (SHK-1) cell line?

Hello, I'm very new to cell culture, so I am trying to determine the best way to culture my cells. I have been trying to culture salmon head kidney (SHK-1) since March and it is growing...

06 June 2023 5,362 1 View

Protocol for BriteVu Perfusion of Mouse Coronary Vasculature?

I am trying to use BriteVu casting reagent to perfuse the coronary vasculature and image using microCT. I have tried 1) perfusion via catheterization of the descending aorta with ligation of...

11 May 2023 2,589 1 View

What theoretical framework is suggested?

What theory would you suggest in a dissertation with the title of: 'Teachers’ Perceptions of Efficacy in Integrating Social-Emotional Learning into the Curriculum for Middle School Gifted Students' ?

06 March 2023 2,927 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Can I Use D-PBS for Preparing a 10% Sucrose Solution for Lentivirus Concentration?

Hello, I am a master's student currently conducting lentivirus transduction experiments in Jurkat cells. The virus is not effectively entering the Jurkat cells, so I am planning to use an...

21 July 2024 4,676 4 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

Stable cell line generation: GOI is detected by qPCR but protein is not expressed?

Hello, I'm generating stable cell lines by transfecting plasmid DNA with lipofectamine 3000. The cells went through antibiotics selection for 3 weeks (polyclonal selection) and when I analyzed...

11 July 2024 4,812 5 View

Why citations are not recorded on my profile researchgate web?

Dear colleges , I would appreciate if you have a time to help. I received mails told me there are numbers for research articles. These citations are not recorded on my profile. Regards, Sherif

09 July 2024 6,195 1 View

Lentivirus transduction of HEK293T cells?

i need to ask that when I transduce my HEK293T cells with Lentivirus, how much time will they require for transduction because when I add lentivirus and then change the media after 24 hours, my...

07 July 2024 2,946 1 View

Can anyone send me the detailed protocol of lentiviral titer determination and its invitro transduction into Hela cell line or MSCs?

I have searched a lot of information from the published article but couldn't find valuable. As I have prepared the Lentiviral vector and stored it in -80 degree!

21 June 2024 3,484 1 View

Over expression in HEK293T cells?

Hello researchers, i need some help, i am packaging Lentivirus( having my transgene) in HEK293T cells, as I will collect the supernatant and concentrate my lentivirus,I also want to check...

15 June 2024 1,596 3 View

Optimal conditions for lentiviral transduction of B-ALL cells (I'm using Nalm6 cells)?

Dear Esteemed Researchers, I am currently waist-deep into the literature to determine the optimal cell density and transduction volume for lentiviral transduction conducted in 24- and 48-well...

10 June 2024 4,167 0 View

Lentiviral Transduction Issues?

Hi there, I am attempting to transduce cells with the TLCV2 plasmid containing our sgRNA of interest. My transfection of the HEK 293T cells seems to yield positive results as the EGFP is clearly...

06 June 2024 2,131 1 View