I want to insert my gene to pCMV6-Entry(Origene company) plasmid by using SfaA I and Mlu I,
Hi, I'm analyzing data from an oncology trial and trying to perform statistical analysis including cox regression analysis and paired t-tests. My data includes laboratory test values, including...
22 June 2024 6,181 1 View
I preprared curcumin-loaded liposome by Thin Film Hydration Method using lecithin, curcumin and beta-sterol. I stained it with phosphotungstic acid and uranium acetate and took TEM images of each....
30 May 2024 6,863 2 View
I have never success a staining pax7, myoD, eMHY on mouse tissue. (DSHB antibody) When I did a heat-retrieval, staining is success but background was too strong. So I'm not sure if this is a good...
27 May 2024 1,597 1 View
Hello, I am experiencing an error while using Discovery Studio and would like to ask for your assistance in identifying the cause. The details of the error are as follows: [ 22-May-2024 14:59:14...
21 May 2024 4,980 0 View
Hello, I am newbie to HFSS and I need to define the periodic boundary conditions on the upper faces of the wave guide that is clarified in the attached file. Could you please let me know how I...
18 May 2024 2,444 0 View
Hi, I get confused about the CST calculations for reflection and transmission values (S- S-Parameters) for different kinds of transmission lines. 1)First could you please let me know where is the...
02 May 2024 7,967 4 View
Hi, In the scheme, I want to define the two above faces as the periodic boundaries on Y axis. But as I defined it, the CST considers the maximum size of the total shape on Y axis as periodic...
02 May 2024 4,573 1 View
Hello, I am in charge of discovering new compound candidates using AI. Currently, we want to create hundreds of thousands of virtual compounds and then select final candidates through...
21 April 2024 9,784 0 View
I want to download a few Nature Cities papers for study, but I can't do it through my school account, is there any way to access them?
15 April 2024 8,376 1 View
I want to make 30 equivalents of concentrated NH4OH using a 30% wt aqueous NH3 solution, referencing the method below. I'm having trouble understanding how to produce equivalents of concentrated...
14 March 2024 6,160 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...
30 July 2024 1,412 8 View
I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?
29 July 2024 3,229 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View