Kehua Jin

3 Questions 5 Answers 0 Followers

Questions related from Kehua Jin

Where is the transcription and translation start site of pCMV6-Entry plasmid?

I want to insert my gene to pCMV6-Entry(Origene company) plasmid by using SfaA I and Mlu I,

27 May 2023 4,003 0 View

Why is my tag gene not detectd?

My target gene fusion with Flag , I detected target protein overexpressed in mammalian cells , but i can not detect Flag. The construct's sequence is correct withou frameshift. What is the reason?

27 May 2023 1,011 4 View

Why the overexpressed protein‘s size is 10 kd larger than the original protein?

pCAG-GFP plasmid contains a chimeric intron and GFP, I cut this plasmid with EcoR I and Not I , so the GFP sequence was removed , then I insert my interested protein(95kd) gene to replace GPF....

03 April 2023 1,668 0 View