I want to screen my samples for telomerase inhibition.
I am planning to isolate cells from frozen xenograft tissues (stored in liquid nitrogen). The tissues are stored in 10% DMSO, 30%FBS and 60% RPMI with Penn/Strep. These tissues were frozen with a...
14 December 2020 3,910 1 View
Hello everyone, I am doing an experiment in which, the plan of work is to electroporate a clone (plasmid form) into chicken spermatozoa, which will be used further for artificial insemination....
07 September 2020 7,855 2 View
I am trying to CG relax a amorphous GeSe system with B3LYP and single K-point (gamma). Even though I have a 250 atom model, the calculations seem to be extremely slow. Is there a way to speed up...
23 August 2020 8,848 4 View
I have a UPLC system and XBridge C18 ( 3x150mm, 3.5 um). I am running short of time and cant procure a UPLC compatible column for the time being.
15 December 2019 8,433 12 View
I need a data set of dorsal hand vein images for experimental purpose.
07 October 2019 1,438 1 View
HI, I am finding difficulty with the solubility of Platinum (II) chloride. I wanted to prepare PtCl2 in water for electrochemical application.
21 August 2019 5,444 3 View
We have derived from certain astrometric and cosmological observations how physical constants evolve in time (https://doi.org/10.3390/galaxies7020055). The theoretical basis used is rather...
25 May 2019 8,899 3 View
Hi everyone, I am looking forward for a solution to my problem. I want to attach an enzyme covalently on gold nanoparticle surface. If i follow EDC-NHS chemistry, the enzyme-nanoparticle complex...
14 April 2019 7,238 2 View
Tartaric acid content by HPLC
09 April 2019 3,675 10 View
washed the column in the reverse direction also but the same problem persists. when flow rate is reduced to 0.8mL/min from 1 ml/min...the pressure reduced to 2800 psi apprx. what more can be done...
04 January 2019 7,935 13 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
