I am in an academic research project, where we want to introduce a gene that codes for obtaining an enzyme that degrades phenols in the organism P. Infestans, so we want to introduce a construct together with the enzyme of interest in the organism, and this It leads me to ask myself the following question: We want to introduce the gene near the ribosomal site since it is a very conserved region and therefore this would prevent the gene from being maintained in the organism, so could this be possible? Or is it better? place the construct in another part of the body's genome?
Greetings.
It's usually difficult to transform fungal cells. You may prepare protoplasm of fungal cells after treatment with Novozyme. Thence expose desired DNA to them preferably by electrporation.
It is possible to insert your gene in a specific region of the fungus genome, but mostly techniques are still not validated. However, if your objective is to introduce a gene that codifies an enzyme that degrades phenols, then Shamsher S. Kanwar already answered your question. The described method might insert your gene of interest "randomly" in the fungus genome. You just have to select a mutant strain that fulfill your research objectives.
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