Where do you get IL-15 antibodies from and how does it work in WB using whole cell lysate and sub-cellular fractions?

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Chintan Koyani

Hi Anil.

You may get IL-15 antibody from abcam.

http://www.abcam.com/il15-antibody-ab7213.html

However, I would suggest to make ELISA over WB bcos cytokines are released by cells into cell culture medium and only a fraction of them you may find in the cell lysates. You may perform ELISA using cell culture medium and/or cell lysate.

All the best,

Chintan

Anil Poudel

Thank you Chintan. My interest is in intra-cellular. I am using RnD for ELISA.