Anil Poudel

3 Questions 24 Answers 0 Followers

Questions related from Anil Poudel

How do I quantify GTP hydrolysis in frozen whole cell lysate?

Anyone with experience of quantifying GTP-bound proteins in frozen lysate? How long these samples are good? I snap froze them. Kit suggests they are good for several months avoiding freeze-thaw...

09 September 2015 3,659 0 View

Where do you get IL-15 antibodies from and how does it work in WB using whole cell lysate and sub-cellular fractions?

Interested in intracelluar proteins not the secreted ones.

06 June 2014 5,694 2 View

Apoptosis assay by flow cytometry?

I am doing flow cytometry for apoptosis and using alexa fluor 488 kit with annexin-v and PI double staining. When I analyze the data from FACScalibur by flowjow do I need to select a sub...

01 January 2014 5,168 17 View