The strain Y258 of yeast was used as the host for expressing the protein because of the deficient in proteases pep 4. Where did the yeast strain Y258 evolution from? What's it the ATCC number?
Does the binding pattern change if the scFv was converted into the Fab or whole antibody? What about other characteristics, such as the affinity, specificity, stability?
05 June 2018 1,674 3 View
In my experiment, the size of dot blot on the NC membrane was different. Sometimes, the coffee ring was formed, in which the signal was less in the centre part. How to resolve this question?
07 August 2017 6,786 0 View
How to improve the expression level of small proteins, such as their MW was less than 20kD?
03 April 2017 7,811 0 View
The rate of glucose consumption by the neocortex is reduced by over 80% during anesthesia (Sibson et al. 1998), which disables the synapses (Richards 2002) that are inundated by glial tissue (Engl...
08 August 2024 3,118 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...
06 August 2024 4,849 2 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...
05 August 2024 1,238 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I attended a lecture at the Baylor College of Medicine (~ 2019) where one of the questions was “Does birdsong have anything to do with human language?” Noam Chomsky would say, “Absolutely not!”...
31 July 2024 1,706 4 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View