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Does the binding pattern change if the scFv was converted into the Fab or whole antibody? What about other characteristics, such as the affinity, specificity, stability?
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In my experiment, the size of dot blot on the NC membrane was different. Sometimes, the coffee ring was formed, in which the signal was less in the centre part. How to resolve this question?
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The strain Y258 of yeast was used as the host for expressing the protein because of the deficient in proteases pep 4. Where did the yeast strain Y258 evolution from? What's it the ATCC number?
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