Hi all,
I'm planning an experiment about host sanctions in domesticated and wild soybean. I'm hoping to use fluorescent-labelled rhizobia in order to quantify colonisation by a competent N-fixing strain, and a non N-fixing strain. USDA 110 is a commonly-used strain for soybean inoculation, so ideally I'd like to use this strain and a near-isogenic mutant with the nifH gene disrupted. Does anyone have, or know anyone else who has such a strain that they'd be able to send me a sample of?
Thanks very much,
-Niall Millar
The answer I believe is yes. The Rhizobium which comes from a green nodule will not have leghemoglobin needed to fix Nitrogen. These will infect the plant but not give any Nitrogen benefit.
The curated bacterial strain of Bradyrhizobium japonicum 110 is a nitrogen fixing wild strain.
Small nonred nodules might yield a variant with genetic inability to fix nitrogen.
Another approach is the use of a mutagenic agent to create nonfixating strains.
Mutant non-fixing strains can be used to determine the nature of the biochemical pathways critical for wild type functioning.
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