27 June 2018 5 5K Report

I would like to purchase commercial hydrophobic PVDF hollow fibre membranes.

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What is the proper dosage for supplemental biotin in primary cell culture?

I am trying to overexpress a TurboID construct in mouse primary culture cells for validation experiments. Unfortunately the transfection of this construct appears to be killing my cells while...

26 February 2024 8,931 2 View

How to measure the DMA peak of Propolyene?

Hello every expert, When I did the DMA expertment for propolyene, I can't not get the normal spectrum. I intended to find the tg peak of PP, but it was not easy to have the beautiful peak in this...

22 February 2024 7,216 0 View

Repeated measures in three groups: which test to use?

Hello, I have an experiment in which three groups of 15 participants (disorder 1, disorder 2, control group) underwent an exercise test. I have 14 measurement points (each with a 5-min interval)....

13 December 2023 1,508 4 View

After qPCR analysis of gene expression, when is difference in relative fold change actually relevant?

During work for my undergrad thesis, I've examined and compared gene expression of stress-induced genes in plants challenged with a fungal infection. I have calculated the relative fold change and...

22 November 2023 5,518 3 View

Mushroom cultivation: can we reduce water needed for entire process while also providing drinking water for billions of people?

Our social enterprise's focus [0] is mushroom cultivation in developing countries where easy access to water cannot be assumed: 20%, globally, still have to walk away from their premise to get it...

31 October 2023 9,856 0 View

Is serum starvation necessary for cell culture?

If I am using growth medium containing BPE instead of FBS, is it still necessary for me to serum starve my cells for 24 hours before subjecting them to drug treatment? My purpose is to study cell...

14 October 2023 2,467 1 View

What is the best way to fix cells for non-permeabilized stains?

Hello, I need to stain some primary culture cells in permeabilized and non-permeabilized conditions to test if a protein is localizing to the exterior of the cell membrane. Our existing fixation...

20 September 2023 5,142 3 View

Why did I get a collection of 5 genes when I search for the gene data of SMN1?

This is what I got when I searched for SMN1 (survival of motor neuron 1) gene: https://www.ncbi.nlm.nih.gov/gene/6606 However, when I downloaded the sequence data, I found there are 5 gene...

14 August 2023 8,123 0 View

Does anyone have any idea on the range of concentration of Gefitinib that causes cell cytotoxicity in NHEK cells?

I'm trying to screen for a suitable range of concentration of Gefitinib for my subsequent ELISA assay, the final aim is to select a Gefitinib concentration that does not kill cells+give off...

31 July 2023 5,130 1 View

Can I expose my drug stock under UV in the biosafety cabinet?

I have prepared my drug stock with filtered-sterilized DMSO outside the biosafety cabinet, can I expose them under UV for a few minutes together with my cell culture tools before further diluting...

16 June 2023 6,805 3 View

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Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

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What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

What is the impact of collaborations with key suppliers on an SME's competitiveness?

The literature on Supply Chain Integration (SCI), Supply Chain Management (SCM), and collaborations, spanning several decades, deserves special attention. This topic, although addressed for a long...

23 July 2024 5,672 3 View

Why is the baseline fibrosis level of HK-2 cells very high?

In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...

15 July 2024 1,929 2 View

How to decrease hydrophobicity of Ni-foam?

As, my studies are related to water adsorption, the substrates should be hydrophilic in nature. Ni-foam being hydrophobic in nature, the results are not as expected as in literature. Hence i...

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Used NIRS equipment?

Hello, Does anyone know where we could purchase a used NIRS system, for example, by Artinis or NIRx? Thank you. Greg

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How to purchase metabolites of pesticide standards ?

Penconazole 1. CGA 132465 2. CGA 190503 3. CGA 127841 Fluopyram 1. AE C656948-benzamide 2. AE C656948-pyridyl-acetic acid 3. AE C656948-carboxylic acid I have searched on sigma aldrich as well...

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After regeneration studies of the PVDF membrane, will the permeate flux and TDS removal efficiency increase or decrease?

Regeneration Studies for the PVDF membrane and PVDF/CuO nanocomposite membrane were carried out by chemical cleaning.

07 July 2024 7,033 0 View

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