I want to purchase DR5(Tnfrsf10b) gene knockout C57BL/6J mice.
I want to do a case control study on the gene polymorphism. now there is a gene on the X chromosome. so it is only one allele of this gene. that is hemizygous. so how to calculate the HW...
10 November 2017 703 3 View
I major in the association of SNPs and risks of some diseases. In my article, Haploview software was conducted to calculate the frequencies of haplotypes formed by several SNPs and the effect of...
05 June 2016 9,905 1 View
I know that the haplotype frequencies can be evaluated by haploview. however, I have no idea about SPSS?
05 June 2016 8,151 3 View
can you help me? i want to search the rs number about 844ins68 loci in cystathionine-β-synthase(CBS) gene in humans,but i can not found it in other reports in publication? thank you!
03 April 2015 9,433 0 View
Compared to the controls, the frequency of FF genotype in VDR gene was decreased (P=0.012), but this P value was merely achieve the statistically significant: after bonferroni correction, the...
31 December 2014 6,116 12 View
i feel uncertain that the two journals of "Genetic testing" and "Genetic Testing and Molecular Biomarkers" are the same one?
11 December 2014 4,245 1 View
a previous study found that there exist more than 200 SNPs in VDR gene.
10 November 2014 2,820 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
