I am looking to analyse banana tissue samples for relative quantities of proteins associated with various metabolic functions. Wherecan I get this done as a fee for service rather than running the samples myself?
Thanks!
Dear All, I found a paper related to my work. I will do the same thing except I will change the reagent they use. For example, instead of using reagent X, I will use reagent Y. They use gold...
25 June 2024 5,799 0 View
There is research into the models individually, but not one seeking to find an overall best one suited for any condition.
23 June 2024 5,641 3 View
I want to perform PacBio RNA-seq of a filamentous fungal isolate during multiple infection time-points in barley leaf. I was told that PacBio RNA-seq would not be viable due to the low...
12 June 2024 4,027 1 View
Hello everyone, My question for sure is silly, but I have been arguing with my labmate about this. If our plasmid concentration is 0.776 µg/µL and he wants to make a final concentration for...
10 June 2024 9,942 5 View
I want to prepare perfusion buffer consisting of: collagenase B (2.5 mg/ml), Dispase II (1.2 U/ml; Roche), Bacillus licheniformis protease 7.5 mg/ml (P5380; Sigma), DNase I (LS002058, 125...
02 May 2024 4,928 0 View
I have designed 2 primers Now I need to set up the PCR protocol. First: I need to know how much of each primer, H20 and GoTaq® G2 Hot Start Taq Polymerase to put in the mix. We usually put a...
28 February 2024 213 2 View
Hello All, I have several questions about qPCR. I will explain what I have done to give you a clear picture. I have 2 kidney samples. 1 wildtype and 1 KO for a specific gene. We have extracted...
25 February 2024 6,331 2 View
I have extracted genomic DNA from 2 tissues. 1 wild type and 1 KO. I havd designed primers that should amplify a region in between the two loxp sites. I want to validate the effectiveness of the...
22 February 2024 9,660 0 View
I have raw qPCR data. 2 samples "1 control and 1 with gene knock out". I made a serial dilution of each sample and performed a qPCR using 2 reference genes and 3 genes of interest. Now I have the...
21 February 2024 2,745 5 View
Hi! Does anyone have ideas to separate Fe(II) complexes from Fe(III) complexes? Context about the complexes: 1) Coordination of the ligand causes some type of Fe(III) to Fe(II) reduction...
07 February 2024 8,464 2 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View