How can I use Permanova to analyze individual variation with RNA seq transcripts and multiway Anova design?

I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...

13 February 2021 7,217 4 View

Does anyone know of an anti-rat CLEC-2 antibody for IHC?

I am looking for an anti-rat CLEC-2 antibody for IHC in rat lungs. Has anyone come across one? There are a few mouse ones but the data sheets don't say they cross react with rat, and the ones that...

06 December 2020 3,511 0 View

Help finding right expression to fit data?

Hello, I have some data that looks like the line on the right, but I'm not sure what function to use. It seems like an exponential added to a line, or something of the like. I think it may be the...

18 November 2020 2,357 3 View

Single-turnover kinetics question?

It is my understanding that single-turnover reactions isolate the chemical step, as opposed to being comprised of the chemical step + product dissociation as in kcat. Here's my issue though....

13 November 2020 2,494 7 View

What are the efficient methods available to test the soil fertility?

Want to understand the scientific mechanisms to know type of soil suitable for agricultural purpose.

22 October 2020 4,941 3 View

Contrasting singular means?

Does anyone know of a method or approach in SPSS to contrast singular mean scores of overall samples, based only on the overall mean value, standard deviation figure and sample size? The data is...

29 September 2020 5,872 6 View

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09 July 2020 9,914 1 View

What are some good recommendations for free software that could be used to quantify video data on juvenile fish behavior and swimming activity?

Ideally I would like to use free software that can be used with a Macintosh. My objective is to quantify individual swimming patterns from 2-dimensional video data. I would like to collect...

16 March 2020 7,635 3 View

Can anyone recommend a journal focused on "assessment & evaluation" in education that looks favourably on qualitative research?

Looking for a place to submit an article related to assessment and evaluation in physical education that was rejected clearly due to being qualitative in nature.

06 February 2020 3,229 2 View

Nuclear staining with a membrane directed antibody?

I have an E-Cadherin antibody. I have been playing around with the parameters for ICC on 293T and NIH3T3 cells. For some reason, i keep getting a lot of nuclear localisation even if i don't...

01 February 2020 1,255 3 View

How to avoid ATP degradation while performing ATP assay?

I am using the ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) for measuring intracellular ATP from treated cell lines. Although my standards are wokring fine when using the fluorometric...

15 February 2021 3,746 4 View

What is the time required for an enzyme or an protein molecule to consume an ATP molecule during a reaction?

In what time do a protein body consumes an ATP molecule for an active process to happen?

02 October 2020 8,135 4 View

What are the effects of phosphorus deficiency on dehydrogenase activity in soil bacteria and fungi?

I suspect that under phosphorus deficiency the electron transport chain in the plasmamembrane and mitochondria of soil bacteria and AM fungi, respectively, is disrupted as a result of lower ATP...

30 August 2020 4,659 3 View

Please recommend biological investigation of newly identified anticancer compounds that specifically inhibit Src kinase?

We aim to publish ta high impact journals (If 6+). Compounds showed incredible activities on cancer cell line (submicromolar on several types). I need your recommendation on how to determine...

24 May 2020 1,230 3 View

Monitoring ATP binding to proteins: TNP-ATP or MANT-ATP?

Hi all, I'm interested on monitoring ATP binding to a protein of interest using fluorescent ATP analogs, such as TNP or MANT-ATP. Do you have any experience with them? Which one is the best...

03 May 2020 3,993 5 View

ATP content measurement?

Hello, I am looking for a clear protocol for ATP content assay. I am not looking for a kit because it is expensive. I am looking for a fluorogenic or a colorimetric substrate that helps me to...

24 March 2020 2,286 3 View

ATP evoked calcium response in skin fibroblasts: no response?

23 March 2020 4,854 2 View

Does POM1 cause precipitation with ATP?

Hi, I've been doing multiple experiments to measure POM1 inhibition of ATP breakdown by CD39. I measure the amount of breakdown using either the malachite green assay or the ATP luciferase...

17 February 2020 5,340 1 View

Is ATP the best energy providing molecule? can there be something better?

ATP energy-storing molecule as is no doubt an efficient! as not only it is used as energy storage and provider in all forms of life and has probably been around for billions of years. Even in the...

08 February 2020 9,015 6 View

How to measure Km for ATP with Kinase Glo Plus?

Dear colleagues, Is there anyone who worked with Kinase Glo Plus reagent? I have to measure Km for ATP for ATP-hydrolyzing enzyme in the range from 0.3 to 100 mcM. Is it possible to do it...

11 December 2019 9,661 2 View