Does anyone know if there is a website where i could get a screen saver of ATP synthase in action?
Thanks
What is the physical significance of critical exponents( Beta, Gamma, and Dealta) at the Curie transition region?
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I am trying to optimize my control titration for ITC so that I can ensure my ligand to protein traces are reliable. My small molecule is stored in DMSO. I have been very careful to avoid buffer...
14 February 2024 9,147 3 View
Hello, I have recently switched from doing ligand into protein for ITC because my ligand is in DMSO and has low solubility at 10% DMSO; therefore, now I am doing protein into ligand -- the heat...
09 February 2024 4,680 1 View
Hello, I am having an ITC issue because I cant increase my ligand concentration due to solubility issues. I know there is past literature on reverse titrations being acceptable. I am more asking...
06 February 2024 5,564 2 View
Hello, I am doing ITC and getting repeatable curves that look very promising. After reading some articles, it seems the biggest challenge to avoid is buffer mismatch. Is there a way to rule out...
18 January 2024 1,224 5 View
Hello, I have had moderate success with ITC but most of my issues have been with the Nano Program in fitting. I have a clear curve but the program is not fitting it correctly, does anyone have a...
22 December 2023 9,331 1 View
Respected all, Please could anyone please guide me as to why I'm not able to install ds visualiser in my windows 11 its always showing error 1935 and rolling back the action. All the chances of...
15 December 2023 1,174 2 View
I want internally labelled RNA oligos at a reasonable price. Could any one help?
20 November 2023 7,996 0 View
Hello, I am doing a titration of ligand into protein and am getting good heat releases compared to my ligand into buffer. I am getting more heat release in later injections. Could cooperativity...
07 November 2023 775 4 View
I would like to know how long it takes to get access after requesting permission from authors for articles.
13 October 2023 530 2 View
Hello everyone We are building an atomic structure model against a cryo-EM map of AAA+ ATPase. Considering that AAA+ polymerizes when ATP is added, we collected data for AAA+ mixed with ATP. Our...
04 April 2024 4,640 2 View
I am trouble shooting a malachite green ATPase assay using a kit from Sigma (MAK307) for a cytosolic protein that converts ATP --> ADP + Pi and am wondering what cheap + commercially available...
30 March 2024 9,659 1 View
Hello! I'm plannning to investigate the relationship between mitochondrial mass/function and epithelial homeostasis/differentiation. Are there any activators(drug) of mitochondria interms of...
27 March 2024 8,273 0 View
I need to order an ATP Assay Kit for my work, and I found this kit on the BT Lab website. Has anyone tried the kits from this site? Because this is the first time I will order from them.
27 February 2024 6,940 1 View
Hello, I performed the ADP-Glo kinase assay. In that case, samples(active protein+substrate+ATP+Kinase buffer+w/wo test compound mixture) were incubated for 45 minutes in 30℃. In the next...
27 December 2023 8,915 2 View
I am working with HepG2 cells to observe effects of Simvastatin treatment on the cells. We are aiming to identify a dose response associated with levels of ATP, metabolic activity, and CoQ10...
29 November 2023 6,062 0 View
I am currently trying to develop an ATP concentration measurement protocol for my human cultured cells. However, I have found little to no detailed protocol for ATP extraction using TCA...
27 November 2023 7,980 1 View
Hi dear All. I'd like to know simplest protocol to detect ATP binding assay (ATP binding with protein). I read a couple of research about this but I want to know common methods. Thanks for your...
19 November 2023 7,412 0 View
process of human obtaining energy in cellular respiration
28 October 2023 7,303 1 View
I’m having issues with the stability of ATP. My lab has purchased multiple brands of ATP (Sigma # A6419; SantaCruz #SC-202040), each of which seem to come hydrolyzed. My lab uses the Malachite...
18 October 2023 3,648 0 View