I've made a Cas9 virus using the #60957 plasmid from Addgene, and I need to titer it using qPCR. There's no GFP reporter, and I'd prefer not to target the AAV ITRs because there are two of them and I've been informed that determining viral titer using the AAV ITRs isn't quite straightforward.
My plan is to design primers targeting Cas9, but I need a Cas9 standard. Does anyone know where I can get an aliquot?