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Questions related from Jane Lindborg
I've been isolating nuclei from mouse retina using a nuclei EZ Prep isolation kit from Sigma, but a huge portion of the nuclei clump together after this process and make sorting impossible. Does...
04 August 2019 1,701 2 View
I just want to know that after in vivo antibody injection, the levels of antibody are higher several hours post-injection compared to several days post-injection. I'm not sure if antibody...
29 July 2019 8,445 3 View
I've generated separate sgRNA-AAVs & Cas9-AAVs and co-transduced neurons. I've looked at DNA editing and protein, but I've gotten the occasional odd result when looking at mRNA expression....
08 September 2018 6,952 4 View
I've made a Cas9 virus using the #60957 plasmid from Addgene, and I need to titer it using qPCR. There's no GFP reporter, and I'd prefer not to target the AAV ITRs because there are two of them...
09 May 2018 6,134 3 View
I'm new to this type of analysis and am unsure of the identification of everything in this image (see attached). This is a 100x image. Is there a clear picture of a phagocytosing macrophage?...
03 July 2015 3,695 5 View
I need the sciatic nerves unfixed for a subsequent assay. Before, I've embedded the nerves in 4% agarose and used a vibratome to cut 60 um sections. However, the entire nerve detaches from the...
12 June 2015 3,694 3 View
I tried incubating the mouse tissue with an unconjugated IgG (for 2 hours) prior to adding the mouse secondary, but I still get a lot of background. Is there a way to reduce the background without...
28 March 2015 4,814 3 View
