Jane Lindborg

6 Questions 6 Answers 0 Followers

Questions related from Jane Lindborg

Any tips for reducing the "stickiness" of nuclei for FACS sorting and downstream applications?

I've been isolating nuclei from mouse retina using a nuclei EZ Prep isolation kit from Sigma, but a huge portion of the nuclei clump together after this process and make sorting impossible. Does...

04 August 2019 1,683 2 View

What's the best way to confirm raised antibody levels in mouse serum after injecting the same antibody?

I just want to know that after in vivo antibody injection, the levels of antibody are higher several hours post-injection compared to several days post-injection. I'm not sure if antibody...

29 July 2019 8,407 3 View

Where can I get a Cas9 virus of known viral titer to use for a standard curve for qPCR?

I've made a Cas9 virus using the #60957 plasmid from Addgene, and I need to titer it using qPCR. There's no GFP reporter, and I'd prefer not to target the AAV ITRs because there are two of them...

09 May 2018 6,082 3 View

Any advice on the details of semi-thin toluidine blue image of an injured sciatic nerve?

I'm new to this type of analysis and am unsure of the identification of everything in this image (see attached). This is a 100x image. Is there a clear picture of a phagocytosing macrophage?...

03 July 2015 3,676 5 View

Any suggestions for using a vibratome to cut unfixed nerves embedded in agarose?

I need the sciatic nerves unfixed for a subsequent assay. Before, I've embedded the nerves in 4% agarose and used a vibratome to cut 60 um sections. However, the entire nerve detaches from the...

12 June 2015 3,673 3 View

How can I reduce the binding of a mouse secondary to IgGs on mouse tissue?

I tried incubating the mouse tissue with an unconjugated IgG (for 2 hours) prior to adding the mouse secondary, but I still get a lot of background. Is there a way to reduce the background without...

28 March 2015 4,794 3 View