Where can I find the RNA sequence of env precursor P14 of mouse mammary tumor virus in 4T1 cells?

More Dibyangana Bhattacharyya's questions See All

How to store fish tissue sample for proximate analysis and antioxidant enzyme analysis?

I need to analyse proximate composition in whole fish and antioxidant enzyme analysis. What is the protocol to store these samples for maximum efficiency in the results. I have -80 degree C...

05 June 2024 1,372 6 View

Acute toxicity test is not showing proper results. Want to know what could be the factors?

When we started the experiment at first at 1ppm all the fishes died within 12 hours. repeated the experiments with lower concentrations starting from 0.05 ppm and found a very good response to the...

02 March 2024 3,097 8 View

How to solve Fuzzy Boundary Value Problem by using Fuzzy Laplace Transform Method?

There are so many papers available to solve initial value problem by using Fuzzy Laplace Transform method. But need solution for the boundary value problem by using the same method.

29 November 2023 9,727 2 View

Can interpretations based on non-parametric tests be accepted for non-normally distributed sample in the fields of Experimental/Survey Research?

For a non-normally distributed small sample (N

20 November 2023 1,953 4 View

When i going to do Rietveld Refinement in X-pert highscore plus it is showing negetive intensities in scan data points.. Why it is showing?

Actually I want to insert hkl values for my peaks in XRD pattern.. hkl values are not coming automatically in x-pert highscore plus software. So i have to do Rietveld Refinement. But when i am...

12 December 2022 8,784 8 View

What's a nice starting reference on calculation of off-diagonal spin correlations using the directed loop (SSE) algorithm?

How should one normalize the answer correctly, after creating a histogram of the separation between the head and tail of a worm?

07 November 2022 6,542 2 View

How to achieve denaturation and renaturation of protein from pellet fraction of the cell (BL21 DE3)?

I am struggling with a Mycobacterium protein (phthalate esterase), which upon overexpression forms inclusion body (maybe) and it is found in the pellet fraction. I have tried cloning the protein...

27 July 2022 2,716 6 View

Lost protein (phthalate hydrolase) activity which I could achieve once but in later attempts the protein is not showing function? Please suggest??

I have cloned a phthalate hydrolase (~37 kDa) into a pMAL-c2x vector and overexpressed in E.coli BL21(DE3). Induction condition - 28 degrees for 3h with 0.5mM IPTG. This is a Mycolicibacterium...

07 June 2022 6,486 4 View

How to calculate protein content per gram of tissue sample in Fish Tissue?

I have used Lowry method to calculate protein content in fish muscle. Briefly, 5% tissue homogenate is prepared in 0.25 M sucrose. Centrifuged and the supernatant is used for analysis. 0.1 mL of...

31 May 2022 6,205 0 View

How metal is extracted from a leached solution?

I was going though this paper "Extraction of vanadium from high calcium vanadium slag using direct roasting and soda leaching" but finally how could they extract from the leached solution I didn;t...

02 September 2021 4,167 3 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Frederic Lepretre

Hi Dibyangana

NCBI is a huge database where you can find whatever you're searching for.

for your request, test this link:

https://www.ncbi.nlm.nih.gov/search/all/?term=MMTV

all the best

fred

Dibyangana Bhattacharyya

Thank you Frederic Lepretre . I’m going to back trace the protein sequence to get the nucleotide sequence and then design a primer set.

Nucleotide sequences are given in the propositions made by NCBI