I need systemic enzymes that reduce mucus membrane. Also, how can I procure mucus from various specimens.
04 May 2015 527 5 View
I need to fix mucus on slides for SEM studies. Does this work on native mucus without adding any chitosan etc? Can anyone suggest other possible options?
03 April 2013 2,410 2 View
Hallo, I am working on dna activity. I need a enzyme that is as slow as possible. I have enzyme which is 500b/min. Is there any thing more slower?? or how can i slow down ezyme activity... if...
31 December 2009 6,450 6 View
I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...
21 February 2021 5,379 2 View
Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...
14 February 2021 2,610 1 View
Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...
12 February 2021 1,386 3 View
Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...
08 February 2021 7,285 1 View
07 February 2021 2,006 1 View
Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...
04 February 2021 6,051 8 View
Dear RG community, We are looking for a fast and easy way to stain mucus on a cell layer that allows for macroscopic visualization of mucus distrubution. Our epithelium is grown in conventional...
31 January 2021 6,790 2 View
I have used potassium ferricyanide (which is likely the most common) extensively in the past, but I am curious to know if there are other oxidants that work well. I need to generate the oxidized...
29 January 2021 9,803 4 View
Hi all, I would like to know how can I estimate the activity of the enzyme, papain, which is used in reactions as crude latex extract of papaya. Please share some procedures if anybody having...
26 January 2021 569 4 View
25 January 2021 5,403 2 View