Hallo,
I am working on dna activity. I need a enzyme that is as slow as possible. I have enzyme which is 500b/min. Is there any thing more slower?? or how can i slow down ezyme activity...
if someone has enzymes which work slower, i can test them too on lot of templates that i have..any help and suggestions are welcome
Hallo Stephen,
It is about pcr synthesis activity, as i have to reduce the rate of synthesis..so i m looking at ways like slowing down enzyme and so...
Thanks for the reply,
Greetings,
Harish.
Dear Harish,
you are working on DNA activity by using PCR technique. you are using enzyme taq polymerase of which company? you want to slow its activity as slow as possible. I will reply you soon after some more discussion on this matter.
Hallo,
i m studying slow down enzyme activity to increase effectivity of my specific reaction as i have eerors with normal pcr. I study mutations and information tansfer.
So you are getting errors in your transcript... Are you just trying to do normal PCR with commercial Taq? What enzyme are you using?
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