Hi All,
recently I was thinking using SYN promoter instead of my CAG promoter to get better GFP express in neuron. Then I searched, I found SYN promoter seq is little bit different between different companys, and also different on addgene. DId anyone know a place which I can find a standard SYN promoter sequencing?
Thanks
Hi:
A paper "Efficient gene transduction of neurons by lentivirus with enhanced neuron-specific promoters" show a detail information about SYN promoter and primer sequences for promoter amplification. Enclosed please find this paper. If you need SYN1 promoter sequence, please check this web site : http://www.ncbi.nlm.nih.gov/nuccore/NC_000086.7?report=fasta&from=20860511&to=20920918&strand=true
You can find promoter sequence from ATGAACTACC. And you can change region shown from 20860511 to 20925918, then you can find around 5000bp SYN promoter sequence.
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