I´m working with a novel protein, we know a few things about it. It has been localized in cytoplasm and nucleus. The crystallographic study of this protein is starting but we want to know also if this protein has a specific receptor.
Hi there,
I think the reading of this article should be helpful:
http://www.nature.com/nprot/journal/v8/n7/full/nprot.2013.072.html
It's about TRICEPS technique which allows to link covalently ligand to its receptor and to affinity purify the covalent complex for further analyses including receptor identificaion.
Hope it will help.
Hi Mercedes, you can do a BLAST of your protein and (hopefully) find an already identified protein homolog.
Then you can run that identified protein through search engines that have compiled protein-protein interactions
http://bioinfow.dep.usal.es/apid/index.htm
http://string-db.org/
http://dip.doe-mbi.ucla.edu/dip/Main.cgi
more protein-protein interaction databases here:
http://mips.helmholtz-muenchen.de/proj/ppi/
thank you, that is the main problem, this protein (cemp1) doesn´t match with any known protein so far.
Hi Mercedes, I found one site that shows that cemp1 (I take it that it is cementum protein-1
http://www.ncbi.nlm.nih.gov/protein/187956787?report=fasta
ineracts with a zinc finger protein and a protein phosphatase:
http://string-db.org/newstring_cgi/show_network_section.pl?taskId=6TxVT5jsrdwt&advanced_menu=_unassigned&interactive=no
Hopes this helps, but I have to update my protein interaction database links since they keep changing.
Also if you run a BLAST of your protein sequence (cementum 1?) in FASTA format you get this. See attached.
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