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Has anyone used the new E1 Clip tip digital pipettes?

We just purchased a multichannel and a single channel E1 Clip tip pipettes. In our first attempt to use them for an experiment they are not dispensing accurately? The rep told me that...

01 July 2024 1,983 0 View

Why would Vmax and Km not change when inhibitor shows clear reduction in activity?

We have been working with an inhibitor that clearly and consistently reduces an enzymes product by about 25-30%. However in trying to elucidate the mechanism using Michaelis-Menten and...

14 April 2024 4,921 5 View

To remove residual ethanol for column purification, would using a desiccator work?

I have been having troubles with column purification (for DNA) recently. I am assuming that the reason is the residual ethanol. I used to centrifuge at max speed for 2 minutes to remove residual...

30 November 2023 3,211 1 View

Can you help with question about MIchaelis Menten graphs for Lysozyme activity?

We have been studying the effects of some inhibitors on lysozyme activity. When we look at a graph of product (fluorescence) over time we see a clear inhibition of the enzyme activity that...

05 November 2023 9,834 1 View

Is it possible to make the voltage of slack bus or PV bus variable in Simulink ?

I want to have a normal distribution of PV or Slack bus voltages in order to calculate the mean and the variance... The results of the simulation always give a constant value for the slack and PV...

11 October 2022 8,203 1 View

How to Isolate Top agar with already phage titre?

I didn't deal with phage methods before. for that, I don't have the experience or the imagination so when I read "Isolate the top agar with the phage titre, then centrifuge" within procedures. I...

10 September 2022 7,777 5 View

Is there published research about the polysaccharide composition of cereal brans?

Hi, I am interested in the polysaccharide composition of wheat and oat bran. I assume that cellulose, arabinoxylans, mixed-linkage glucans and possibly some starch will be present, because these...

06 July 2022 4,882 0 View

Can we replaced PEG by any other fusible material when we preparing solid dispersion by melting-solvent method?

I have to replace it and I want to make sure I'm still using the same method

09 December 2021 8,152 3 View

How i can interprete ANOVA info in SPSS?

Dear professionals, i run ANOVA test in SPSS and I found that three independent variables were not significant. I exclude them and re-run the test again ANOVA Test. the result are almost the...

25 October 2021 4,005 4 View

Are the Micrococcus lysodeikticus cells in the enz check lysozyme assay intact?

We have been using the Enz Check lysozyme assay and are assuming the cells are not intact but in pieces. However over time and especially with vortexing or pipetting to mix substrate, I would...

02 October 2021 3,491 1 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

Why does everyone use vs code?

Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...

07 August 2024 7,013 4 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View