Where can I download full genomes of cancer cell lines?

More Bastian Fromm's questions See All

To what extent is an Other constructed through a certain representation of disability?

For example, when the villain is visualized with a physical mark.

08 May 2024 8,857 0 View

How are people with disabilities represented in fictional films?

Here I am primarily concerned with the construction of an alien other using the example of the horror film.

08 May 2024 3,745 2 View

What effect do representations of disability have on the concept of abjection?

08 May 2024 5,434 3 View

Do certain representations and visualizations create ableism?

Does this create and produce certain ableist stereotypes?

08 May 2024 2,284 0 View

Organ-on-a-chip material procurement?

Hello, I would like to establish an experimental setup for a generic two-organ-on-a-chip (OoC) system. Can the material (chamber, pump,...) needed for this be purchased commercially? Best...

20 December 2022 8,313 0 View

Multinomial logit or what model is better?

I observe if persons, who are asked questions, will ignore them, respond verbally only, respond nonverbally only or respond verbally and nonverbally. Dependent on three dummy variables for type...

13 December 2022 9,013 1 View

How can one obtain the same-spin and opposite-spin terms of the MP2 energy by integrating out the spin?

In the Moller-Plesset Perturbation Theory, the second order correction to the ground state energy can be divided into a same-spin and an opposite-spin terms by integrating the spin out. This...

01 December 2022 1,335 1 View

Is there a difference between strain hardening and work hardening?

Dear engineers, scientists and experts in the field of plasticity, In the literature, the terms strain hardening and work hardening are often used under the theory of istropic hardening. Mostly...

31 October 2018 560 27 View

Feeder cell separation?

Dear all, I am doing some CRISPR experiments in neonatal fibroblasts. After a lot of optimization, the system works really well in these cells. As you will know, fibroblasts don´t really like to...

10 October 2018 1,270 0 View

Cloning gRNA into pSpCas9(BB)-2A-Puro (Addgene)?

Dear all, I've successfully used gRNA's that target the top DNA strand, but now I'd like to use a gRNA that targets the bottom strand. I just wanted to confirm that my cloning strategy is correct...

15 January 2017 5,862 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Jonas Cicenas

http://cancergenome.nih.gov/, http://www.completegenomics.com/public-data/cancer-data/, https://cghub.ucsc.edu/datasets/ccle.html, http://www.sanger.ac.uk/science/tools/cancer-genome-browser... stuff like that... Nothing perfect, though...

Shangwei Ning

The UCSC Cancer Genomics Hub (CGHub,

https://cghub.ucsc.edu/), try it!

It may help you!