Does anyone have experience with nattokinase, or another way to dissolve fibrogen?

We want to extract RNA from a 3D TC and nattokinase was suggested, but i am not sure whats the best supplier - dissolving technique, and amount and incubation time to add to the 3D TC ?

01 September 2020 7,424 3 View

Problems to open VIS files with ImageJ?

I want to open vis files that were created with the Olympus slide scanner vs120 by FIJI (imageJ) with the plugin olympusviewer. The funny thing is that most files can be opened properly, but some...

26 March 2020 3,123 1 View

Resazurin cell viability assay possible with fluorescent samples?

Hey everyone, I did a resazurin assay in a 96 well plate using HEK293 cells and measured the fluorescent signal over time (every hour for the first 6 hours, at 24 and 48 h). I think the handling...

08 February 2018 697 3 View

Is it valid to compare qPCR data collected from two different instruments for the same study?

I am beginning a project that will require me run upwards of 1000 individual full qPCR plates to measure the natural variation in gene copy number (mito genes). I have access to two departmental...

02 February 2018 195 1 View

Can i change pH of buffer after cleaving Hyaluronan with Hyaluronidase?

For an experiment i need the small cleavage products of Hyaluronan, meaning that i need especially the activity of Hyal-1 in the product H3884 of Sigma. I guess this is why standard protocols use...

25 April 2017 2,804 2 View

How can I internalize proteins (NO DNA) into living bacteria (E. Coli) ?

Hello, I would like to study the effect of adding a certain protein to living E. coli to study it's effect, not by producing the protein, but and I have to deliver it by internalization. I DON'T...

23 April 2017 1,428 1 View

Help with cancer cell spheroid formation and 3D invasion assay?

I have recently began culturing a melanoma cell line in hanging drop plates to form spheroids with the intention of harvesting the spheres into the bottom of a 96-well round bottom plate and...

13 September 2016 144 3 View

(Spatial) Working memory task in headfixed mice ?

I am investigating (spatial) working memory in mice. Due to the nature of the project the mice are head-fixed and therefore are not able to move around freely, eliminating most common tests for...

04 July 2016 2,997 2 View

After addition of protease inhibitor protein concentration (Bradford) decreased when sample was centrifuged but increased when filtered. Why is that?

03 July 2016 1,475 11 View

What is a good program to draw mitochondrial genomes?

I am looking for a good and free to use program to draw circular mitochondrial genomes from a file (not genbank). thanks in advance

04 February 2016 4,979 5 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

How do I collect online measures in a same-different task?

The same-different task requires to subjects to indicate whether a pair of stimuli seen or heard are the same (say AA or BB) or different (say AB or BA). Researchers often collect offline measures...

20 February 2021 9,226 2 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

Can I get the design of circular cylinder to fix thermocouple on the surface and cartridge heater in core ?

Respected Scientific Community. I'm interested to carry out experimental studies on a circular cylinder. But even after doing literature review I unable to get the exact design of the cylinder...

10 February 2021 6,221 1 View

What are the applicable reinforcement learning based control methods for systems that have control delay?

I am studying the experimental application of reinforcement learning (RL) based control methods. For that reason, I modeled a nonlinear system without delay and trained the RL agent. In this...

02 February 2021 7,999 3 View

Where to buy optically clear, low viscosity, refractive index 1.5-1.4 Silicone Oil ?

For research purposes, it is required to have optically clear, low viscosity silicone oil with a refractive index of 1.5-1.4. It is going to be used in optoelectronic research. Online purchase is...

01 February 2021 9,160 3 View

The litter effect and what can I do to avoid it?

My thesis involves experimental study with Wistar puppies. I am dividing the puppies equally in relation to the groups. However I only have 4 mothers and I will use all of their puppies. There is...

25 January 2021 6,965 3 View

Lupasol G100 from BASF -- where to buy?

Hello everyone. I am looking to purchase Lupasol G100 (essentially ~5kDa bPEI) for creation of carbon dots for in planta transient gene expression. Thus far, I have only been able to find a...

20 January 2021 7,683 1 View

Can we use the single-elimination tournament method as the basis of an experiment design?

I want to conduct an experiment in the field of Human-Computer Interaction, where I test the perception of users about which image is better using different camera lenses. In the experiment, I...

18 January 2021 779 2 View