What is the general cyclic energy degradation factor for plain concrete at material level in stress strain curve?

Plain Concrete subjected to cyclic loading get reduction in its stiffness and strength with the every cycle of loading. What is ratio of energy enclosed in one hysteresis loop to adjacent...

10 February 2021 1,051 1 View

The Akaike information criterion of the random forest model ?

Please, how to calculate the number of model parameters of the random forest model to determine the Akaike Information Criterion (AIC) using this following expression: AIC = n * log(MSE) + 2 *...

27 January 2021 816 6 View

Suggest me some satellite image datasets used for crop yield estimation?

I have my synopsis presentation and i have to provide details about 2-3 datasets that I can use in my research. Topic is crops Yield estimation using satellite images. suggest me what I can write?...

13 January 2021 1,675 3 View

What is the reason of suddenly increasing the concentration while evaluating the photocatalytic performance by RhB dye degradation?

I am doing a photocatalytic performance evaluation by RhB dye degradation. I am facing a problem which is: After achieving the absorption -desorption equilibrium, I checked the concentration and...

04 December 2020 3,922 20 View

Edu assay by spectrophotometry means?

Dear all, Have any one performed edu assay by spectrophotometric means ? I'm looking for a kit for that purpose. I have Chinese kit for this purpose, I followed their protocol but the result is...

24 November 2020 1,766 3 View

Either SEM analysis are enough to justify the morphology and micro structure of activated carbons? or I should go for TEM analysis as well?

While I was discussing my nitrogen adsorption isotherms in the context of micropore, mesopores, total pore volume for activated carbon samples prepared from waste materials, I received a review...

13 November 2020 1,971 3 View

How can we measure dissimilarities between two images layout? Like alignment of button, text box, text overlapping?

I want to extract dissimilarity information of two images layout. Wanna check text, button, and text box alignment, text overlapping, and more other layouts difference. how we do that? any tool,...

25 October 2020 7,190 7 View

Any suggestions of bioinformatic tools to identify/predict the effect of mutations observed on the function of proteins?

04 October 2020 1,552 4 View

How to Determine LD50 of a test compound by Lorke's Method if your mice (mus musculus) dies at the lowest concetration i.e. 10mg/kg?

I have used Lorke's method of determining LD50 of my test drug. The lowest dose according to this method is 10mg/kg and my mice died at this dose within the 30 minutes of IP injection. Does that...

22 September 2020 9,959 3 View

How to do MTT for non-confluent cells?

Hi everyone, I'm doing MTT assay to test the cytotoxicity of 4 candidates on the BT-474 breast cancer cell line which rarely becomes confluent. started with 3K/well, 5K/well, 6.5K/well left the...

12 September 2020 3,034 8 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

In frequency domain alpha band(8-16hz), beta band(16-32hz), gamma band(32-64hz), sub-band(64-128hz) are same as LL,LH,HL,HH in wavelet decomposition?

I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...

23 February 2021 5,503 1 View

MVN, how to conciliate contradictory results from Multivariate normality and Multivariate outliers test?

Dear colleagues, I'm working with Path analyses in lavaan and MVN packages. There are some results that for me are confusing and features from the MVN that I do not know how to set. My dataset is...

18 February 2021 190 3 View

Is it possible to obtain a negative cronbach alpha?

Good evening everyone. If a questionnaire has 4 factors measuring different constructs as for example CAMI questionnaire measuring attitudes toward the mentally ill that has four factors:...

18 February 2021 3,223 3 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View