I've been using genomic screen tape on our agilent tapestation to compare DNA length in different samples, but it's really inconsistent. I get wildly different results for the same sample. I was chatting to a guy at a conference and he said he was having the same trouble. Does anyone else have experience of this, or can suggest what's going wrong?
I was having the same trouble. The tapestation and bioanalyzer series is not pricise to evaluate the length of DNA. I think that this problem may be caused by the qality and preservation of the gel after adding the dye.
When loading the same sample on the tapestation, multiple times. I'm afraid I have no other method for length analysis- our bioanalyser doesn't go up that high and we don't have a PFGE system. Annoyingly, I just have to trust the tapestation.
Someone suggested the problem may be "daisy chaining". The DNA strands appear to get longer as the ends stick to each other. Has anyone else come across this?
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