I am confused about selecting of enzyme for a Southern Blot and where exactly to cut it. I am working with knock out and used hygromycin selectable marker.
Thank you for your answer, do you mean that enzyme should cut in ORF but not on hph?
I am thinking about conservative site PCR product. When PCR products were amplified by universal primer ITS 1and ITS4 region of rDNA, the products length differ among Candida species. Although the...
04 May 2019 1,570 4 View
Does anybody have information regarding using 3D electrophoresis in the studying gene expression? Does this method could analyse the expression of all genes in the organism or it target specific...
27 November 2018 8,234 3 View
Hi I have new sequence and I want to download in the NCBI website and get reference number. Does anybody can share the steps of this process? Regards Ayat
06 July 2018 7,977 0 View
I got some minus value in the my results, I repeated the experiment three time, and get same values. Does anybody could advice me that how can I treated them statistically? Thank you
18 May 2017 5,531 10 View
04 May 2017 2,568 11 View
I want to count killing percentage by using MTT assay. I incubated spores with macrophage in time course and used MTT dye for overnight, dye concentration 0.1 mg/ml. My question is that I want to...
15 August 2016 7,438 2 View
07 August 2016 7,801 2 View
Dear all, I am trying to count alive spore from dead one after incubation with macrophage by using flow cytometer, Does anybody has an idea, how can I select suitable label to my spores and...
16 July 2016 7,817 5 View
06 July 2016 10,104 5 View
I want to lysate macrophage after co-incubation. Does anybody share protocol to lysis macrophage? I know, It could be lysis by dipping in the liquid nitrogen but I want protocol does not affect...
29 May 2016 968 5 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar
29 July 2024 3,084 8 View