Mostly the incubation time of bacteria is 48 hours. But, if i want to know the dry mass of the bacteria. So, how long i should incubated it? is it 24 hours, 48 hours, or in the mid log phase? because i use minimal medium for my bacteria.
Thankyou
Dear Irma Suci
Pls. find the attached files
https://www.boundless.com/microbiology/textbooks/boundless-microbiology-textbook/culturing-microorganisms-6/counting-bacteria-63/measurements-of-microbial-mass-385-7951/
http://www.eng.umd.edu/~nsw/ench485/lab9c.htm
https://www.researchgate.net/profile/Roland_Psenner/publication/13767278_Determination_of_Bacterial_Cell_Dry_Mass_by_Transmission_Electron_Microscopy_and_Densitometric_Image_Analysis/links/00b4952cc4fc83a77b000000.pdf
http://www.dot.ca.gov/hq/esc/ctms/pdf/CT_226.pdf
http://manalis-lab.mit.edu/publications/delgado_cermak_PLOS_2013.pdf
http://vlab.amrita.edu/?sub=3&brch=73&sim=1105&cnt=1
http://w3.ualg.pt/~hgalvao/mpob/methodsmicrobiolvol30_1-2/sdarticle_013.pdf
http://www.int-res.com/articles/meps/70/m070p073.pdf
http://www.nature.com/nprot/journal/v4/n5/full/nprot.2009.37.html
http://www.curtislab.org/research-resources/electronic/cell-dry-weight-protocol
Hoping this will be helpful
Regards
Prof. Houda Kawas
Article Determination of Bacterial Cell Dry Mass by Transmission Ele...
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