How to compute the refraction index for a 79.5GHz (3.8mm) FMCW Radar Instrument?

Dear everyone, I performed some experiements with a FMCW Radar Instrument emitting waves of 3.8mm. I can see some "movements" when I am looking on the relative displacements (+/- 0.15mm) over...

07 January 2021 2,073 6 View

Diazonium salt reagents - better to synthesize or purchase?

Are any organic/catalytic chemists able to weigh in? I am starting a project that requires deposition of diazonium salts onto the surface of nanoparticles, and am trying to figure out the best...

01 September 2020 4,562 7 View

Experience in using the LiDAR of the new iPad Pro for mapping?

Has anyone experience in using the LiDAR of the new iPad and if so, what App have you used? We want to map fault structures or rather fractures of damage zones and I wonder if this can easily be...

01 August 2020 7,473 4 View

Maleimide dye labelling of Ni2+ NTA resin?

Hi. When I purify my His-tagged membrane protein with IMAC, I label the protein (containing a single cysteine) with fluorescein-5-maleimide while the protein is immobilized to the Ni2+ NTA. I...

20 July 2020 2,065 3 View

Is anyone familiar with making a new cell line available for everyone? How to have ATCC or someone else to sell your cell line?

We have developed a new cell line and would like to make it available to everyone. Is anybody familiar with this process? What are the requirements? Are there other companies other than ATCC that...

19 May 2020 1,390 7 View

2-way ANOVA - splitting data or not?

Hi. My question is about 2-way ANOVA (and Freidmans test). I've no problem with the test per se but rather with the dataset in relation to the test(s). My problem is that I've 16 different...

06 April 2020 6,920 4 View

Why information criteria differ so i cant compare arma models?

Hello, i am trying to forecast using eviews the stock price returns of a specific company. I am running automatic arima forecasting so as eviews evaluates and proposes the best model fit...

30 March 2020 9,431 4 View

Paper for using PROCESS for mediation with two independent variables?

14 October 2019 356 3 View

How to stoichiometry calculate this combustion process?

Hi! This one is for you chemists/chemical engineers out there. I have a fuel, tall oil pitch, that I want to burn mixed with ordinary air in an industrial burner for heat generation. The tall...

01 October 2019 5,143 12 View

How to dissolve CaWO4?

Hi, What is the best way to dissolve pure CaWO4? I tried: - conc. Aqua Regia - conc. Aqua Regia + conc. HF - conc. HNO3 + conc. HF - 7N HNO3 - 7N HNO3 + conc HF. Every experiment was for 55...

29 September 2019 5,982 1 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View