I'm not sure whether the Interleukin gene name is IL-4 or IL4 and whether the protein is IL-4 or
IL4. Can anyone help?
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
19 July 2024 8,522 0 View
15 July 2024 2,414 1 View
When preparing hierarchical zinc oxide ananostructure, the energy gap measured by PL was equal to (3.26 eV) While the energy gap value was determined by UV-Vis measurements (3.5 to 3.7 eV)
26 June 2024 7,505 6 View
Hi, I am running an opensees model of a 2-bay 1-storey frame with settlement at the mid-support. I wanted to add an additional frame element as a bracing to the analyzed frame after it sustains...
08 June 2024 4,132 0 View
I just found many protocols but not specific for bacterial growth
30 May 2024 6,305 2 View
I have three nanogels.. The difference between them is the cationic monomer ratio... However, the test is giving me similar curve of the three formulations
27 May 2024 2,064 0 View
Hello, I was elaborating thin films from FeCl3 using a dip coater. These films were deposited on a glass sheet for 6 layers and then annealed at 500°C. However, XRD analysis did not yield any...
31 March 2024 9,413 3 View
I am trying to express m6A protein and a fluorescence one RFP in the piggyback vector. I was thinking first of using the P2A to separate between the m6A and the RFP protein but then I was afraid...
12 March 2024 9,713 0 View
I have synthesised a 100 nm sized cationic nanogel and I want to use it as a carrier for cisplatin and paclitaxel
03 March 2024 3,426 0 View
Recently, I tried to differentiate THP1 into M2 macrophage using IL4 and IL13 (purchased from R&D). This is my protocol. THP1 was seeded into 6-well plate (10E6 cells/well). Incubate with PMA...
15 July 2024 5,153 2 View
I am attempting to culture tissue resident T cells (TRM) derived from fresh intestinal tissue for up to six days. I am finding that the viability of the T cells is low at D0, and a very small...
08 July 2024 4,887 2 View
Can
08 July 2024 6,725 4 View
Hello everyone, I am isolating PBMCs and stimulating them using different stimulation conditions. my question is, I want to add IL-2 to the medium for better viability after stimulation, Would you...
16 June 2024 928 1 View
I'm performing interactions between DNA as the macromolecule and Ethidium Bromide as the ligand. I'm looking to make the entire DNA molecule flexible in order to perform blind docking. However, I...
10 June 2024 6,270 1 View
Hi all, I am working on a project which needs expansion of CD45+ selected cells from tumor and lymph nodes. My Tumor samples expand good but my samples from the lymph nodes are less and less...
23 May 2024 2,839 0 View
What percentage of IL-17 positive cells are expected to be positive in Th17-polarized cells in flow cytometry? Day-1: Coating anti-CD3 and anti-CD28 Day 0: Seeding of purified CD4 cells +...
15 May 2024 7,056 0 View
As we know the devastating outcome in Children with SRSE looking for further insights from researchers across the world as what they are trying to get better outcomes for these Children Is Early...
11 May 2024 4,713 0 View
Currently, there are numerous blood biomarkers that have been linked to fractures and increased fracture risk (for example, IL-6). Considering the current evidence, which ones do you think are the...
10 May 2024 6,097 2 View
Hello everyone. I have a question regarding CFSE proliferation assay with mice. My goal is to see CD3, CD4, and CD8 populations. Why do I keep getting one peak, as if there is no proliferation? I...
08 May 2024 7,744 0 View