Scopus Index Journal List , 2021?

please provide me the most recent Scopus Index Journals List, February-2021

27 February 2021 3,545 1 View

By mistake I created another account and I want to add the published papers in the new account into the old one and cancel the new one?

I made anew account because I was invited be one of my coauthors and I forgot my password by then

28 December 2020 6,409 2 View

Pretrained networks with Adam optimizer?

I have tried different pretrained networks with Adam optimizer, and obtained high performance. However, when I tried it with Alex network, the performance was poor. Could you please help me...

28 September 2020 5,621 1 View

May anyone help me to insert binary data into Popgene correctly?

Hello everyone, I am trying to use Popgene 32 software for analysis of binary data for dominant marker of five ISSR primers across five populations and having 38 loci. Unfortunately the program...

22 September 2020 7,369 4 View

Which Tm should I use in my PCR ?

I have ordered primers from Thermo fisher scientific and in the analysis sheet it has been mentioned two tm The first one is tm 1M na+ 70 and the second is Tm 50mM na+ 48 and each has different...

29 August 2020 6,890 12 View

How to peel off PDMS film from a glass petri dish without damaging it ??

I have prepared 15wt.% PDMS and poured it on a glass petri dish, then let it dry for 72 hr at room temp. after I tried to peel it and it doesn't work, so I am crosslinked in an oven for 3 hr , but...

26 July 2020 9,892 2 View

What is the best solvent for PVDF ?? Dimethyl sulphoxide or Dimethylacetamide??

For membrane preparation PVDF will be used , and I was wondering which solvent is the best for dissolving it, non-Toxic solvent , useful in dissolving other polymers such as PES, PDMS, Peba

05 July 2020 1,655 5 View

May anyone recommend me a source for learning R programming language and R packages for biological research disciplines?

I want to learn R programming language but I do not know a source from which I can start, I need it for statistical analysis of Ecological, Molecular, floristic, and Taxonomic studies.

14 June 2020 4,985 9 View

Why fluorescamine don't exhibit fluorescence by itself?

it has groups that allow it to be fluorescent but it doesn't

10 April 2020 6,483 3 View

For einstein equation (E=MC^2) it means that what have mass have energy ?

but photon doesn't have or it is negligible and it could have huge amount of energy . HOW?

10 April 2020 3,545 14 View

Do you have any experience with transfection of EBV immortalized B cells with Lipofectamine?

I know that efficiency of transfection for primary B cells is very low with Lipofectamine. But I did not try that for EBV immortalized B cells. Do you have any experience with transfection of EBV...

05 February 2021 4,159 1 View

What is the best concentration of Pam3CSK4 to coat a dish to activate with THP-1?

Hi everyone I am trying to coat a glass-bottom dish with Pam3CSK4 and plate THP-1 cells to measure IL6 with ELISA from the supernatant. I wondered if anyone knows a good concentration and...

21 January 2021 2,593 1 View

How to specify binding sites in AutoDock Raccoon?

Hi all, There are two big questions here. I am extremely grateful for even the smallest contribution that yields a result. This is concerning Raccoon(1) original, not Raccoon2. The original...

17 December 2020 4,302 2 View

How to run AutoDockTool's Autogrid4 command?

Hi all, Please imagine this to be a brand new machine with the latest installation of: Autodock Vina Autodock AutodockTools (MGLTools). I'm following a Nature Protocol for the prediction of...

14 December 2020 2,191 3 View

Best purification method for recombinant protein in BL21?

Hi I need to purify a recombinant protein (IL6 21kDa). It's been produced in E.coli BL21 with a T7 expression vector, but it does not have a tag as yet. I was thinking of adding a his-tag and...

09 December 2020 2,200 8 View

ELISA detection cytokine in human serum (common dilution fold)?

Hi everyone, I'm gonna try to detect the IL-10, IFN-y, TGF-beta1 level in the human serum by ELISA. I bought the ELISA set from the BD contain Capture Ab, Detection Ab, Enzyme reagent, Standards....

09 December 2020 3,369 5 View

Greiner Bio or Corning Costar plates for ELISA?

09 December 2020 237 2 View

Recombinant Interleukin 6 western blot, non specific bands?

Hi I've been trying to produce some recombinant human Interleukin 6. I sub-cloned the gene for human IL6 into a Promega T7 Flexi vector (native gene no tag yet) and confirmed sequencing and...

09 December 2020 2,340 4 View

How to run Autodock 4 , using CONDA environment on server ?

#autodock #virtualscreening Question1: Am I doing this right , means do setting up conda on server works for virtual screening (AUTODOCK)? Question2: How can I modify the script (submit4.py)...

06 December 2020 2,424 3 View

How to detect TGFb and IL10 from activated Tregs?

Dear colleagues! I plan to check cytokine production by my WT and KO Tregs following activation, particularly, IL10 and TGFb (TGFb1 or LAP, exactly) as these are reported to be the major...

02 December 2020 9,424 5 View