I have seen protocols use either mCSF or GM-CSF so I am unsure which one is better to use.
I have experience culturing peritoneal macrophages and bone-marrow derived macrophages for which I used mCSF.
I have only ever used GM-CSF along with IL-4 to culture bone marrow derived dendritic cells.
Its depending, as both MCSF and GMCSF promotes certain change in the phenotype, probable is better do not use any of them to keep macs in a non activated state. But, for example, if you will treat them with M1 or M2 inducers you should use the proper factor. But if you need that macrophage to live longer (more than 4 days) in culture, you can use a low concentration of GMCSF (2-5 ng/ml).
Thank you, this is helpful Francisco Triana-Martinez . I am looking to culture the alveolar macrophages long term, so I will plan on using a lower concentration of GMCSF as you suggest.
Do you have experience culturing murine interstitial macrophages? If so, did you find using a low concentration of GMCSF to also be beneficial for culturing this macrophage population?
Not really, but I have been also working with peritoneal, fat, and liver (F4/80+) and they have very similar behavior with this approach
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