Olivia B. Parks

7 Questions 7 Answers 0 Followers

Questions related from Olivia B. Parks

How do I dissolve OTX015 in sunflower oil?

I diluted OTX015 to 50mg/mL in DMSO and then diluted that further to 50mg/kg in sunflower oil to administer to mice via oral gavage. Prior to gavaging, I vortexed the OTX015/sunflower oil, but...

04 August 2022 4,251 0 View

How do I perform an in vitro co-culture with macrophages and T cells?

I am trying to set up an in vitro experiment where I co-culture peritoneal macrophages and T cells together in the presence of HMPV infection. After incubating the macrophages and T cells...

23 November 2021 5,087 3 View

Are there any suggestions for how to anesthetize aged B6 mice with age range 70-80wks (with my current protocol I usually kill at least one mouse)?

I infected mice using 3.5% isoflurane in oxygen for about 5min or until their respirations have slowed to 1 second in between breaths. I then quickly infect the mice with 100uL of my virus in...

09 July 2021 1,584 3 View

How long should I co-culture activated macrophages and naive T cells to assess the ability of macrophages to activate T cells?

I am stimulating macrophages with LPS for 24hrs prior to co-culturing with T cells in a 1:10 ratio (1 macrophage: 10 T cells).

21 June 2021 2,901 1 View

How long should I stimulate macrophages with LPS before co-culturing with T cells to assess macrophage ability to activate T cells?

This is a methodology question for a new experiment I am trying.

19 June 2021 1,388 2 View

When culturing murine alveolar macrophages is it better to supplement culture media with mCSF or GM-CSF?

I have seen protocols use either mCSF or GM-CSF so I am unsure which one is better to use. I have experience culturing peritoneal macrophages and bone-marrow derived macrophages for which I used...

15 February 2021 237 4 View

Did I dilute my supernatant too much so cytokines will be below the limit of detection on a Luminex assay?

I am performing an experiment analyzing the cytokine production of macrophages in response to viral infection. I recently harvested and cultured peritoneal macrophages from mice. I plated these...

18 January 2021 5,803 3 View