Olivia B. Parks

3 Questions 4 Answers 0 Followers

Questions related from Olivia B. Parks

How do I perform an in vitro co-culture with macrophages and T cells?

I am trying to set up an in vitro experiment where I co-culture peritoneal macrophages and T cells together in the presence of HMPV infection. After incubating the macrophages and T cells...

23 November 2021 5,037 3 View

Are there any suggestions for how to anesthetize aged B6 mice with age range 70-80wks (with my current protocol I usually kill at least one mouse)?

I infected mice using 3.5% isoflurane in oxygen for about 5min or until their respirations have slowed to 1 second in between breaths. I then quickly infect the mice with 100uL of my virus in...

09 July 2021 1,527 3 View

Did I dilute my supernatant too much so cytokines will be below the limit of detection on a Luminex assay?

I am performing an experiment analyzing the cytokine production of macrophages in response to viral infection. I recently harvested and cultured peritoneal macrophages from mice. I plated these...

18 January 2021 5,686 3 View