Hi, I want to extract RNA from environmental biofilms (water distribution systems) and I am looking for a good protocol. We use FLOQSwabs(TM) and the Fast DNA Spin Kit for soil from MP Bio to extract total DNA and it works very fine. For RNA I'd like to put the swabs into liquid nitrogen directly after sampling and prior to extraction. Does anyone has more experience with it and can give me some tips and advice on sampling and sample preparation? Thanks a lot in advance.
Hi, Rene,
Placella et al. used a method originally introduced by Griffiths et al. with the modifications of DeAngelis et al. , It works well for soil. you may try this method.
Placella et al., Rainfall-induced carbon dioxide pulses result from
sequential resuscitation of phylogenetically
clustered microbial groups. PNAS, 2012,109:10931-10936.
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